Polymorphism in transporter antigen peptides gene (TAPI) associated with atopy in Tunisians

Awatef Ismaïl, Raoudha Bousaffara, Jannet Kaziz, Jamel Zili, Ali El Kamel, M. Tahar Sfar, Sami Remadi, Lotfi Chouchane

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Transporter antigen peptide 1 (TAPI) and TAP2 gene products form a transporter molecule involved in antigen presentation. Polymorphic residues have been described in both genes and could have functional consequences in the immune response. Objective: We designed a case-control study to investigate the potential association of polymorphism of the TAPI gene with atopy. Methods: We used the amplification refractory mutation system polymerase chain reaction to characterize TAPI gene polymorphism in 84 unrelated Tunisian patients with atopy and 81 healthy control subjects. Results: Analysis of TAPI polymorphism in Tunisian patients with atopy and in unaffected control subjects demonstrates a high relative risk (RR) of atopy in carriers of a codon (d) corresponding to a glycine at position 637 of the TAP1-B and TAP1-D alleles. The relative risk of allergic asthma is markedly higher in homozygotes (did) (RR = 22; p ≤ 0.0001). The TAP1-D allele, not observed in European populations, has a frequency of 5% in the Tunisian control subject group. A major increase of the frequency (f) of the D allele is observed in patients with allergic asthma (f = 35%) and in those with allergic rhinitis (f = 22%), indicating a high relative risk of allergic asthma (RR = 10.2; p ≤ 0.0001) and of allergic rhinitis (RR = 5.4; p ≤ 0.005) in individuals carrying this allele. DD homozygotes were found only among patients with allergic asthma (23% of patients with asthma). Further evidence of the strong association between TAP1 polymorphism and atopy was provided by the finding that atopy is transmitted by inheritance of the glycine-637 marker. Conclusions: Tunisian persons carrying the glycine-637 of the TAPI protein may have an increased risk of atopy. Specific association was found between the homozygous TAPI DID genotype and allergic asthma.

Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume99
Issue number2
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Antigens
Asthma
Genes
Alleles
Homozygote
Glycine
Glycine Plasma Membrane Transport Proteins
peptide permease
Antigen Presentation
Codon
Case-Control Studies
Healthy Volunteers
Genotype
Polymerase Chain Reaction
Control Groups
Peptides
Mutation
Population
Proteins
Allergic Rhinitis

Keywords

  • Allergic asthma
  • Amplification refractory mutation system polymerase chain reaction
  • Atopy
  • TAP1 polymorphism

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ismaïl, A., Bousaffara, R., Kaziz, J., Zili, J., El Kamel, A., Sfar, M. T., ... Chouchane, L. (1997). Polymorphism in transporter antigen peptides gene (TAPI) associated with atopy in Tunisians. Journal of Allergy and Clinical Immunology, 99(2), 216-223.

Polymorphism in transporter antigen peptides gene (TAPI) associated with atopy in Tunisians. / Ismaïl, Awatef; Bousaffara, Raoudha; Kaziz, Jannet; Zili, Jamel; El Kamel, Ali; Sfar, M. Tahar; Remadi, Sami; Chouchane, Lotfi.

In: Journal of Allergy and Clinical Immunology, Vol. 99, No. 2, 1997, p. 216-223.

Research output: Contribution to journalArticle

Ismaïl, A, Bousaffara, R, Kaziz, J, Zili, J, El Kamel, A, Sfar, MT, Remadi, S & Chouchane, L 1997, 'Polymorphism in transporter antigen peptides gene (TAPI) associated with atopy in Tunisians', Journal of Allergy and Clinical Immunology, vol. 99, no. 2, pp. 216-223.
Ismaïl A, Bousaffara R, Kaziz J, Zili J, El Kamel A, Sfar MT et al. Polymorphism in transporter antigen peptides gene (TAPI) associated with atopy in Tunisians. Journal of Allergy and Clinical Immunology. 1997;99(2):216-223.
Ismaïl, Awatef ; Bousaffara, Raoudha ; Kaziz, Jannet ; Zili, Jamel ; El Kamel, Ali ; Sfar, M. Tahar ; Remadi, Sami ; Chouchane, Lotfi. / Polymorphism in transporter antigen peptides gene (TAPI) associated with atopy in Tunisians. In: Journal of Allergy and Clinical Immunology. 1997 ; Vol. 99, No. 2. pp. 216-223.
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abstract = "Background: Transporter antigen peptide 1 (TAPI) and TAP2 gene products form a transporter molecule involved in antigen presentation. Polymorphic residues have been described in both genes and could have functional consequences in the immune response. Objective: We designed a case-control study to investigate the potential association of polymorphism of the TAPI gene with atopy. Methods: We used the amplification refractory mutation system polymerase chain reaction to characterize TAPI gene polymorphism in 84 unrelated Tunisian patients with atopy and 81 healthy control subjects. Results: Analysis of TAPI polymorphism in Tunisian patients with atopy and in unaffected control subjects demonstrates a high relative risk (RR) of atopy in carriers of a codon (d) corresponding to a glycine at position 637 of the TAP1-B and TAP1-D alleles. The relative risk of allergic asthma is markedly higher in homozygotes (did) (RR = 22; p ≤ 0.0001). The TAP1-D allele, not observed in European populations, has a frequency of 5{\%} in the Tunisian control subject group. A major increase of the frequency (f) of the D allele is observed in patients with allergic asthma (f = 35{\%}) and in those with allergic rhinitis (f = 22{\%}), indicating a high relative risk of allergic asthma (RR = 10.2; p ≤ 0.0001) and of allergic rhinitis (RR = 5.4; p ≤ 0.005) in individuals carrying this allele. DD homozygotes were found only among patients with allergic asthma (23{\%} of patients with asthma). Further evidence of the strong association between TAP1 polymorphism and atopy was provided by the finding that atopy is transmitted by inheritance of the glycine-637 marker. Conclusions: Tunisian persons carrying the glycine-637 of the TAPI protein may have an increased risk of atopy. Specific association was found between the homozygous TAPI DID genotype and allergic asthma.",
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AU - Bousaffara, Raoudha

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AU - El Kamel, Ali

AU - Sfar, M. Tahar

AU - Remadi, Sami

AU - Chouchane, Lotfi

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AB - Background: Transporter antigen peptide 1 (TAPI) and TAP2 gene products form a transporter molecule involved in antigen presentation. Polymorphic residues have been described in both genes and could have functional consequences in the immune response. Objective: We designed a case-control study to investigate the potential association of polymorphism of the TAPI gene with atopy. Methods: We used the amplification refractory mutation system polymerase chain reaction to characterize TAPI gene polymorphism in 84 unrelated Tunisian patients with atopy and 81 healthy control subjects. Results: Analysis of TAPI polymorphism in Tunisian patients with atopy and in unaffected control subjects demonstrates a high relative risk (RR) of atopy in carriers of a codon (d) corresponding to a glycine at position 637 of the TAP1-B and TAP1-D alleles. The relative risk of allergic asthma is markedly higher in homozygotes (did) (RR = 22; p ≤ 0.0001). The TAP1-D allele, not observed in European populations, has a frequency of 5% in the Tunisian control subject group. A major increase of the frequency (f) of the D allele is observed in patients with allergic asthma (f = 35%) and in those with allergic rhinitis (f = 22%), indicating a high relative risk of allergic asthma (RR = 10.2; p ≤ 0.0001) and of allergic rhinitis (RR = 5.4; p ≤ 0.005) in individuals carrying this allele. DD homozygotes were found only among patients with allergic asthma (23% of patients with asthma). Further evidence of the strong association between TAP1 polymorphism and atopy was provided by the finding that atopy is transmitted by inheritance of the glycine-637 marker. Conclusions: Tunisian persons carrying the glycine-637 of the TAPI protein may have an increased risk of atopy. Specific association was found between the homozygous TAPI DID genotype and allergic asthma.

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