Platelet function following induced hypoglycaemia in type 2 diabetes

H. Kahal, A. Aburima, B. Spurgeon, K. S. Wraith, A. S. Rigby, T. Sathyapalan, E. S. Kilpatrick, K. M. Naseem, Stephen Atkin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aim: Strict glycaemic control has been associated with an increased mortality rate in subjects with type 2 diabetes (T2DM). Here we examined platelet function immediately and 24 hours following induced hypoglycaemia in people with type 2 diabetes compared to healthy age-matched controls. Methods: Hyperinsulinaemic clamps reduced blood glucose to 2.8 mmol/L (50 mg/dl) for 1 hour. Sampling at baseline; euglycaemia 5 mmol/L (90 mg/dl); hypoglycaemia; and at 24 post clamp were undertaken. Platelet function was measured by whole blood flow cytometry. Results: 10 subjects with T2DM and 8 controls were recruited. Platelets from people with T2DM showed reduced sensitivity to prostacyclin (PGI2, 1 nM) following hypoglycaemia. The ability of PGI2 to inhibit platelet activation was significantly impaired at 24 hours compared to baseline in the T2DM group. Here, inhibition of fibrinogen binding was 29.5% (10.3–43.8) compared to 50.8% (36.8–61.1), (P < 0.05), while inhibition of P-selectin expression was 32% (16.1–47.6) vs. 54.4% (42.5–67.5) (P < 0.05). No significant changes in platelet function were noted in controls. Conclusion: Induced hypoglycaemia in T2DM enhances platelet hyperactivity through impaired sensitivity to prostacyclin at 24 hours.

Original languageEnglish
JournalDiabetes and Metabolism
DOIs
Publication statusAccepted/In press - 1 Jan 2018

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Hypoglycemia
Type 2 Diabetes Mellitus
Epoprostenol
Blood Platelets
P-Selectin
Platelet Activation
Fibrinogen
Blood Glucose
Flow Cytometry
Mortality

Keywords

  • Hypoglycaemia
  • Platelets
  • Type 2 diabetes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Kahal, H., Aburima, A., Spurgeon, B., Wraith, K. S., Rigby, A. S., Sathyapalan, T., ... Atkin, S. (Accepted/In press). Platelet function following induced hypoglycaemia in type 2 diabetes. Diabetes and Metabolism. https://doi.org/10.1016/j.diabet.2018.04.004

Platelet function following induced hypoglycaemia in type 2 diabetes. / Kahal, H.; Aburima, A.; Spurgeon, B.; Wraith, K. S.; Rigby, A. S.; Sathyapalan, T.; Kilpatrick, E. S.; Naseem, K. M.; Atkin, Stephen.

In: Diabetes and Metabolism, 01.01.2018.

Research output: Contribution to journalArticle

Kahal, H, Aburima, A, Spurgeon, B, Wraith, KS, Rigby, AS, Sathyapalan, T, Kilpatrick, ES, Naseem, KM & Atkin, S 2018, 'Platelet function following induced hypoglycaemia in type 2 diabetes', Diabetes and Metabolism. https://doi.org/10.1016/j.diabet.2018.04.004
Kahal H, Aburima A, Spurgeon B, Wraith KS, Rigby AS, Sathyapalan T et al. Platelet function following induced hypoglycaemia in type 2 diabetes. Diabetes and Metabolism. 2018 Jan 1. https://doi.org/10.1016/j.diabet.2018.04.004
Kahal, H. ; Aburima, A. ; Spurgeon, B. ; Wraith, K. S. ; Rigby, A. S. ; Sathyapalan, T. ; Kilpatrick, E. S. ; Naseem, K. M. ; Atkin, Stephen. / Platelet function following induced hypoglycaemia in type 2 diabetes. In: Diabetes and Metabolism. 2018.
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