Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells

Koichi Hattori, Beate Heissig, Kei Tashiro, Tasuku Honjo, Masatoshi Tateno, Jae Hung Shieh, Neil R. Hackett, Mannix S. Quitoriano, Ronald Crystal, Shahin Rafii, Malcolm A S Moore

Research output: Contribution to journalArticle

431 Citations (Scopus)

Abstract

The chemokine, stromal cell-derived factor-1 (SDF1), is produced in the bone marrow and has been shown to modulate the homing of stem cells to this site by mediating chemokinesis and chemotaxis. Therefore, it was hypothesized that elevation of SDF1 level in the peripheral circulation would result in mobilization of primitive hematopoietic stem and progenitor cells. SDF1 plasma level was increased by intravenous injection of an adenoviral vector expressing SDF1α (AdSDF1) into severe combined immunodeficient mice. This resulted in a 10-fold increase in leukocyte count, a 3-fold increase in platelets, and mobilization of progenitors, including colony-forming units-granulocyte-macrophage to the peripheral circulation. In addition, AdSDF1 induced mobilization of cells with stem cell potential, including colony-forming units in spleen and long-term reconstituting cells. These data demonstrate that overexpression of SDF1 in the peripheral circulation results in the mobilization of hematopoietic cells with repopulating capacity, progenitor cells, and precursor cells. These studies lay the foundation for using SDF1 to induce mobilization of hematopoietic stem and progenitor cells in in vivo studies.

Original languageEnglish
Pages (from-to)3354-3360
Number of pages7
JournalBlood
Volume97
Issue number11
DOIs
Publication statusPublished - 1 Jun 2001
Externally publishedYes

Fingerprint

Chemokine CXCL12
Hematopoietic Stem Cells
Stem cells
Plasmas
Stem Cells
Granulocyte-Macrophage Progenitor Cells
SCID Mice
Macrophages
Chemotaxis
Platelets
Leukocyte Count
Chemokines
Intravenous Injections
Bone
Blood Platelets
Spleen
Bone Marrow

ASJC Scopus subject areas

  • Hematology

Cite this

Hattori, K., Heissig, B., Tashiro, K., Honjo, T., Tateno, M., Shieh, J. H., ... Moore, M. A. S. (2001). Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells. Blood, 97(11), 3354-3360. https://doi.org/10.1182/blood.V97.11.3354

Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells. / Hattori, Koichi; Heissig, Beate; Tashiro, Kei; Honjo, Tasuku; Tateno, Masatoshi; Shieh, Jae Hung; Hackett, Neil R.; Quitoriano, Mannix S.; Crystal, Ronald; Rafii, Shahin; Moore, Malcolm A S.

In: Blood, Vol. 97, No. 11, 01.06.2001, p. 3354-3360.

Research output: Contribution to journalArticle

Hattori, K, Heissig, B, Tashiro, K, Honjo, T, Tateno, M, Shieh, JH, Hackett, NR, Quitoriano, MS, Crystal, R, Rafii, S & Moore, MAS 2001, 'Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells', Blood, vol. 97, no. 11, pp. 3354-3360. https://doi.org/10.1182/blood.V97.11.3354
Hattori, Koichi ; Heissig, Beate ; Tashiro, Kei ; Honjo, Tasuku ; Tateno, Masatoshi ; Shieh, Jae Hung ; Hackett, Neil R. ; Quitoriano, Mannix S. ; Crystal, Ronald ; Rafii, Shahin ; Moore, Malcolm A S. / Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells. In: Blood. 2001 ; Vol. 97, No. 11. pp. 3354-3360.
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