Picosecond laser cross - linking histones to DNA in chromatin

implication in studying histones - DNA interactions

D. Angelov, S. Dimitrov, E. Keskinova, I. Pashev, V. Russanova, Yu Stefanovsky

Research output: Contribution to journalArticle

Abstract

A picosecond UV laser radiation was used to cross - link proteins to DNA in nuclei, whole cells and different chromatin preparations. All histones as well as high - mobility group 1 proteins were identified immunochemically in the covalently linked protein - DNA complexes. Irradiation of the nucleohistone resulted in cross - linking 20% of bound histones to DNA as a result of two - quantum photoreaction with a maximum quantum yield 3.10-4 for double stranded DNA. When nuclei, total hromatin, H1 - depleted chromatin and core particles were irradiated and then trypsinized or treated with clostripain to cleave respectively the N-, C- and N- terminal histone tails, no histones were found covalently linked to DNA. However whilst the yield of cross - links was similar in total and H1 - depleted chromatin in core particles the efficiency was 3 - 4 times lower for H2A, H2B and H4 and 10 - 12 times lower for H3.

Original languageEnglish
Pages (from-to)230-239
Number of pages10
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1403
Issue numberpt 1
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

chromatin
Chromatin
Histones
Linking
DNA
deoxyribonucleic acid
Laser
Protein
Nucleus
Lasers
Interaction
lasers
clostripain
UV Laser
proteins
Proteins
Laser Radiation
interactions
Irradiation
Tail

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Picosecond laser cross - linking histones to DNA in chromatin : implication in studying histones - DNA interactions. / Angelov, D.; Dimitrov, S.; Keskinova, E.; Pashev, I.; Russanova, V.; Stefanovsky, Yu.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1403, No. pt 1, 1991, p. 230-239.

Research output: Contribution to journalArticle

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