Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

Khaled Mahmoud, Sabahudin Hrapovic, John H T Luong

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/ gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.

Original languageEnglish
Pages (from-to)1051-1057
Number of pages7
JournalACS Nano
Volume2
Issue number5
DOIs
Publication statusPublished - 1 May 2008
Externally publishedYes

Fingerprint

protease
Single-walled carbon nanotubes (SWCN)
Viruses
Gold
Peptides
human immunodeficiency virus
peptides
Peptide Hydrolases
carbon nanotubes
gold
Nanoparticles
nanoparticles
Electrodes
electrodes
Electrochemical impedance spectroscopy
Sulfhydryl Compounds
Cyclic voltammetry
Raman spectroscopy
Electron microscopes
electrode materials

Keywords

  • Biosensor
  • Carbon nanotubes
  • Ferrocene-pepstatin inhibitor
  • Gold nanoparticles
  • Protease

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode. / Mahmoud, Khaled; Hrapovic, Sabahudin; Luong, John H T.

In: ACS Nano, Vol. 2, No. 5, 01.05.2008, p. 1051-1057.

Research output: Contribution to journalArticle

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