Physicochemical and biological characterization of long-term operated sulfate reducing granular sludge in the SANIreg process

Tianwei Hao, Jinghai Luo, Li Wei, Hamish Mackey, Rulong Liu, Guillermo Rey Morito, Guang Hao Chen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The SANIreg process (Sulfate reduction, Autotrophic denitrification and Nitrification Integrated) is a treatment system with low energy demands. The major bioreactor of this new technology is a sulfate-reducing up-flow sludge bed (SRUSB) that converts organics and provides electron donors for subsequent autotrophic denitrification. This research characterizes the granules inside the SRUSB, with the aim of improving its efficiency, maximizing its operational flexibility, and minimizing its footprint. The unique sulfate-reducing bacteria (SRB) granules serving in the SRUSB were found to increase the resilience and compactness of the SRUSB. The granules, with a compact and porous structure, showed high cohesion resisting breakage with a shear force G>3400s-1. The hydrophobicity of the external surface of the mature granules remained stable at around 70% and acid volatile sulfide (AVS) accumulated at the bottom of the SRUSB. 16s rRNA gene analysis of the microbial communities revealed that Desulfobulbus (42.1%), Prosthecochloris (19%) and Trichococcus (12%) dominated the mature granular sludge. Fluorescence in situ hybridization (FISH) further showed that SRB organisms were located internally and then surrounded by non-SRB. According to the FISH results, the spatial distribution of extracellular polymeric substances (EPS) displayed protein and α-polysaccharides in the exterior and β-polysaccharide in the core of the granules. Such biological structure suggests that each SRB granule acts as an efficient and independent unit, capable of achieving both fermentation and organic conversion. The present investigation sheds light on the physicochemical and biological characteristics of the SRB granulate. This information provides valuable information for scaling-up the SANIreg process to treat real saline sewage in Hong Kong.

Original languageEnglish
Pages (from-to)74-84
Number of pages11
JournalWater Research
Volume71
DOIs
Publication statusPublished - 5 Mar 2015
Externally publishedYes

Fingerprint

Sewage
Sulfates
sludge
sulfate-reducing bacterium
sulfate
Bacteria
polysaccharide
denitrification
fluorescence
Denitrification
Polysaccharides
Fluorescence In Situ Hybridization
biological characteristics
hydrophobicity
breakage
cohesion
footprint
bioreactor
Fluorescence
fermentation

Keywords

  • Granular sludge characterization
  • Microbial community and diversity
  • SANI process

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution
  • Ecological Modelling
  • Medicine(all)

Cite this

Physicochemical and biological characterization of long-term operated sulfate reducing granular sludge in the SANIreg process. / Hao, Tianwei; Luo, Jinghai; Wei, Li; Mackey, Hamish; Liu, Rulong; Rey Morito, Guillermo; Chen, Guang Hao.

In: Water Research, Vol. 71, 05.03.2015, p. 74-84.

Research output: Contribution to journalArticle

Hao, Tianwei ; Luo, Jinghai ; Wei, Li ; Mackey, Hamish ; Liu, Rulong ; Rey Morito, Guillermo ; Chen, Guang Hao. / Physicochemical and biological characterization of long-term operated sulfate reducing granular sludge in the SANIreg process. In: Water Research. 2015 ; Vol. 71. pp. 74-84.
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