Physical and genetic analysis of cosmids from the vicinity of the cystic fibrosis locus

P. J. Scambler, X. Estivill, G. Bell, M. Farrall, C. Mclean, R. Newman, P. F.R. Little, P. Frederick, K. Hawley, B. J. Wainwright, R. Williamson, N. J. Lench

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Abstract

Cosmid libraries have been constructed from DNA of somatic cell hybrid cell lines, each containing a fragment of human chromosome seven and including sequences closely linked to cystic fibrosis (CF). Cosmids containing human DNA as insert were isolated from the library. Three cosmids, when used as probes to total genomic DNA, detected polymorphic loci, each of which was shown to be in strong linkage disequilibrium with CF. Restriction endonuclease digestion of cosmid clones and use of a new, rapid method of chromosome walking based on competitive hybridisation of cosmid inserts has allowed identification of several groups of overlapping cosmids ("contigs") from the vicinity of CF.

Original languageEnglish
Pages (from-to)3639-3652
Number of pages14
JournalNucleic acids research
Volume15
Issue number9
DOIs
Publication statusPublished - 11 May 1987

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ASJC Scopus subject areas

  • Genetics

Cite this

Scambler, P. J., Estivill, X., Bell, G., Farrall, M., Mclean, C., Newman, R., Little, P. F. R., Frederick, P., Hawley, K., Wainwright, B. J., Williamson, R., & Lench, N. J. (1987). Physical and genetic analysis of cosmids from the vicinity of the cystic fibrosis locus. Nucleic acids research, 15(9), 3639-3652. https://doi.org/10.1093/nar/15.9.3639