Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U

Maya Ameyar-Zazoua, Mouloud Souidi, Lauriane Fritsch, Philippe Robin, Audrey Thomas, Ali Hamiche, Piergiorgio Percipalle, Slimane Ait-Si-Ali, Annick Harel-Bellan

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Abstract

By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.

Original languageEnglish
Pages (from-to)27974-27979
Number of pages6
JournalJournal of Biological Chemistry
Volume284
Issue number41
DOIs
Publication statusPublished - 9 Oct 2009

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ameyar-Zazoua, M., Souidi, M., Fritsch, L., Robin, P., Thomas, A., Hamiche, A., Percipalle, P., Ait-Si-Ali, S., & Harel-Bellan, A. (2009). Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U. Journal of Biological Chemistry, 284(41), 27974-27979. https://doi.org/10.1074/jbc.M109.037929