Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U

Maya Ameyar-Zazoua, Mouloud Souidi, Lauriane Fritsch, Philippe Robin, Audrey Thomas, Ali Hamiche, Piergiorgio Percipalle, Slimane Ait-Si-Ali, Annick Harel-Bellan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.

Original languageEnglish
Pages (from-to)27974-27979
Number of pages6
JournalJournal of Biological Chemistry
Volume284
Issue number41
DOIs
Publication statusPublished - 9 Oct 2009
Externally publishedYes

Fingerprint

Heterogeneous-Nuclear Ribonucleoprotein U
RNA-Binding Proteins
Chromatin
Gene Silencing
Purification
Mass spectrometry
heterochromatin-specific nonhistone chromosomal protein HP-1
Mass Spectrometry
Proteins
Genes
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U. / Ameyar-Zazoua, Maya; Souidi, Mouloud; Fritsch, Lauriane; Robin, Philippe; Thomas, Audrey; Hamiche, Ali; Percipalle, Piergiorgio; Ait-Si-Ali, Slimane; Harel-Bellan, Annick.

In: Journal of Biological Chemistry, Vol. 284, No. 41, 09.10.2009, p. 27974-27979.

Research output: Contribution to journalArticle

Ameyar-Zazoua, M, Souidi, M, Fritsch, L, Robin, P, Thomas, A, Hamiche, A, Percipalle, P, Ait-Si-Ali, S & Harel-Bellan, A 2009, 'Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U', Journal of Biological Chemistry, vol. 284, no. 41, pp. 27974-27979. https://doi.org/10.1074/jbc.M109.037929
Ameyar-Zazoua, Maya ; Souidi, Mouloud ; Fritsch, Lauriane ; Robin, Philippe ; Thomas, Audrey ; Hamiche, Ali ; Percipalle, Piergiorgio ; Ait-Si-Ali, Slimane ; Harel-Bellan, Annick. / Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 41. pp. 27974-27979.
@article{32dea6b1df92452ab40f92900f1609e5,
title = "Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U",
abstract = "By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.",
author = "Maya Ameyar-Zazoua and Mouloud Souidi and Lauriane Fritsch and Philippe Robin and Audrey Thomas and Ali Hamiche and Piergiorgio Percipalle and Slimane Ait-Si-Ali and Annick Harel-Bellan",
year = "2009",
month = "10",
day = "9",
doi = "10.1074/jbc.M109.037929",
language = "English",
volume = "284",
pages = "27974--27979",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "41",

}

TY - JOUR

T1 - Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U

AU - Ameyar-Zazoua, Maya

AU - Souidi, Mouloud

AU - Fritsch, Lauriane

AU - Robin, Philippe

AU - Thomas, Audrey

AU - Hamiche, Ali

AU - Percipalle, Piergiorgio

AU - Ait-Si-Ali, Slimane

AU - Harel-Bellan, Annick

PY - 2009/10/9

Y1 - 2009/10/9

N2 - By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.

AB - By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.

UR - http://www.scopus.com/inward/record.url?scp=70350463532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350463532&partnerID=8YFLogxK

U2 - 10.1074/jbc.M109.037929

DO - 10.1074/jbc.M109.037929

M3 - Article

VL - 284

SP - 27974

EP - 27979

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 41

ER -