Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice

J. F. Powers, M. J. Evinger, P. Tsokas, Shahinaz Bedri, J. Alroy, M. Shahsavari, A. S. Tischler

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Transplantable tumors and cell lines have been developed from pheochromocytomas arising in mice with a heterozygous knockout mutation of the neurofibromatosis gene, Nf1. Nf1 encodes a ras-GTPase-activating protein, neurofibromin, and mouse pheochromocytoma (MPC) cells in primary cultures typically show extensive spontaneous neuronal differentiation that may result from the loss of the remaining wild-type allele and defective regulation of ras signaling. However, all MPC cell lines express neurofibromin, suggesting that preservation of the wild-type allele may be required to permit the propagation of MPC cells in vitro. MPC lines differ from PC12 cells in that they express both endogenous phenylethanolamine N-methyltransferase (PNMT) and full-length PNMT reporter constructs. PNMT expression is increased by dexamethasone and by cell-cell contact in suspension cultures. Mouse pheochromocytomas are a new tool for studying genes and signaling pathways that regulate cell growth and differentiation in adrenal medullary neoplasms and are a unique model for studying the regulation of PNMT expression.

Original languageEnglish
Pages (from-to)309-320
Number of pages12
JournalCell and Tissue Research
Volume302
Issue number3
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Phenylethanolamine N-Methyltransferase
Neurofibromatoses
PC12 Cells
Knockout Mice
Pheochromocytoma
Neurofibromin 1
Cells
Genes
ras GTPase-Activating Proteins
Cell growth
Neurofibromatosis 1 Genes
Adrenal Gland Neoplasms
Alleles
Brain Stem Neoplasms
Dexamethasone
Tumors
Suspensions
Primary Cell Culture
Tumor Cell Line
Cell Differentiation

Keywords

  • Mouse (knockout mutation of NF1)
  • Neurofibromatosis
  • Neurofibromin
  • Phenylethanolamine-N-methylt ransferase
  • Pheochromocytoma
  • Silencing
  • Transcription

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Powers, J. F., Evinger, M. J., Tsokas, P., Bedri, S., Alroy, J., Shahsavari, M., & Tischler, A. S. (2000). Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice. Cell and Tissue Research, 302(3), 309-320.

Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice. / Powers, J. F.; Evinger, M. J.; Tsokas, P.; Bedri, Shahinaz; Alroy, J.; Shahsavari, M.; Tischler, A. S.

In: Cell and Tissue Research, Vol. 302, No. 3, 2000, p. 309-320.

Research output: Contribution to journalArticle

Powers, JF, Evinger, MJ, Tsokas, P, Bedri, S, Alroy, J, Shahsavari, M & Tischler, AS 2000, 'Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice', Cell and Tissue Research, vol. 302, no. 3, pp. 309-320.
Powers JF, Evinger MJ, Tsokas P, Bedri S, Alroy J, Shahsavari M et al. Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice. Cell and Tissue Research. 2000;302(3):309-320.
Powers, J. F. ; Evinger, M. J. ; Tsokas, P. ; Bedri, Shahinaz ; Alroy, J. ; Shahsavari, M. ; Tischler, A. S. / Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice. In: Cell and Tissue Research. 2000 ; Vol. 302, No. 3. pp. 309-320.
@article{7fc1b84da1b14236a0a3703ba6c9f2de,
title = "Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice",
abstract = "Transplantable tumors and cell lines have been developed from pheochromocytomas arising in mice with a heterozygous knockout mutation of the neurofibromatosis gene, Nf1. Nf1 encodes a ras-GTPase-activating protein, neurofibromin, and mouse pheochromocytoma (MPC) cells in primary cultures typically show extensive spontaneous neuronal differentiation that may result from the loss of the remaining wild-type allele and defective regulation of ras signaling. However, all MPC cell lines express neurofibromin, suggesting that preservation of the wild-type allele may be required to permit the propagation of MPC cells in vitro. MPC lines differ from PC12 cells in that they express both endogenous phenylethanolamine N-methyltransferase (PNMT) and full-length PNMT reporter constructs. PNMT expression is increased by dexamethasone and by cell-cell contact in suspension cultures. Mouse pheochromocytomas are a new tool for studying genes and signaling pathways that regulate cell growth and differentiation in adrenal medullary neoplasms and are a unique model for studying the regulation of PNMT expression.",
keywords = "Mouse (knockout mutation of NF1), Neurofibromatosis, Neurofibromin, Phenylethanolamine-N-methylt ransferase, Pheochromocytoma, Silencing, Transcription",
author = "Powers, {J. F.} and Evinger, {M. J.} and P. Tsokas and Shahinaz Bedri and J. Alroy and M. Shahsavari and Tischler, {A. S.}",
year = "2000",
language = "English",
volume = "302",
pages = "309--320",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice

AU - Powers, J. F.

AU - Evinger, M. J.

AU - Tsokas, P.

AU - Bedri, Shahinaz

AU - Alroy, J.

AU - Shahsavari, M.

AU - Tischler, A. S.

PY - 2000

Y1 - 2000

N2 - Transplantable tumors and cell lines have been developed from pheochromocytomas arising in mice with a heterozygous knockout mutation of the neurofibromatosis gene, Nf1. Nf1 encodes a ras-GTPase-activating protein, neurofibromin, and mouse pheochromocytoma (MPC) cells in primary cultures typically show extensive spontaneous neuronal differentiation that may result from the loss of the remaining wild-type allele and defective regulation of ras signaling. However, all MPC cell lines express neurofibromin, suggesting that preservation of the wild-type allele may be required to permit the propagation of MPC cells in vitro. MPC lines differ from PC12 cells in that they express both endogenous phenylethanolamine N-methyltransferase (PNMT) and full-length PNMT reporter constructs. PNMT expression is increased by dexamethasone and by cell-cell contact in suspension cultures. Mouse pheochromocytomas are a new tool for studying genes and signaling pathways that regulate cell growth and differentiation in adrenal medullary neoplasms and are a unique model for studying the regulation of PNMT expression.

AB - Transplantable tumors and cell lines have been developed from pheochromocytomas arising in mice with a heterozygous knockout mutation of the neurofibromatosis gene, Nf1. Nf1 encodes a ras-GTPase-activating protein, neurofibromin, and mouse pheochromocytoma (MPC) cells in primary cultures typically show extensive spontaneous neuronal differentiation that may result from the loss of the remaining wild-type allele and defective regulation of ras signaling. However, all MPC cell lines express neurofibromin, suggesting that preservation of the wild-type allele may be required to permit the propagation of MPC cells in vitro. MPC lines differ from PC12 cells in that they express both endogenous phenylethanolamine N-methyltransferase (PNMT) and full-length PNMT reporter constructs. PNMT expression is increased by dexamethasone and by cell-cell contact in suspension cultures. Mouse pheochromocytomas are a new tool for studying genes and signaling pathways that regulate cell growth and differentiation in adrenal medullary neoplasms and are a unique model for studying the regulation of PNMT expression.

KW - Mouse (knockout mutation of NF1)

KW - Neurofibromatosis

KW - Neurofibromin

KW - Phenylethanolamine-N-methylt ransferase

KW - Pheochromocytoma

KW - Silencing

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0033674787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033674787&partnerID=8YFLogxK

M3 - Article

C2 - 11151443

AN - SCOPUS:0033674787

VL - 302

SP - 309

EP - 320

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 3

ER -