PGE2 reduces net water and chloride absorption from the rat colon by targeting the Na+/H+ exchanger and the Na+K+2Cl- cotransporter

Rawad Hodeify, Sawsan Ibrahim Kreydiyyeh

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2 Citations (Scopus)

Abstract

An effect of PGE2 on water and chloride absorption was already established in a previous work. This study is an attempt to find the mechanism of action of the prostaglandin by investigating the involvement of three major transporters namely the Na+-K+ATPase, the Na+/H+ exchanger and the Na+K+2Cl- cotransporter. Rats were injected with PGE2 and 15 min later, the colon was perfused in situ with Krebs Ringer buffer, and net water and chloride absorption were determined. When the involvement of the cotransporter and/or the exchanger was investigated, animals were injected with, respectively, furosemide and amiloride 10 min before PGE2. Superficial and crypt colonocytes were then isolated and the protein expression of the Na+-K+ ATPase and the Na+K+2Cl- was determined by western blot analysis. The effect of PGE2 on the pump activity in presence or absence of the transporters' inhibitors was also studied. PGE2 decreased net water and chloride absorption from the colon, increased the Na+-K+ ATPase activity in superficial cells and reduced it in crypt cells. The prostaglandin was found to stimulate secretion in superficial cells by targeting the Na+K+2Cl- symporter, and reduce absorption in crypt cells by targeting the Na+/H+ antiporter. Changes in the activity of the pump are secondary to changes in the activity of the other transporters.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume76
Issue number5
DOIs
Publication statusPublished - 1 May 2007
Externally publishedYes

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Sodium-Hydrogen Antiporter
Dinoprostone
Rats
Chlorides
Colon
Water
Prostaglandins
Pumps
Symporters
Amiloride
Furosemide
Adenosine Triphosphatases
Buffers
Animals
Western Blotting
sodium-translocating ATPase
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

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abstract = "An effect of PGE2 on water and chloride absorption was already established in a previous work. This study is an attempt to find the mechanism of action of the prostaglandin by investigating the involvement of three major transporters namely the Na+-K+ATPase, the Na+/H+ exchanger and the Na+K+2Cl- cotransporter. Rats were injected with PGE2 and 15 min later, the colon was perfused in situ with Krebs Ringer buffer, and net water and chloride absorption were determined. When the involvement of the cotransporter and/or the exchanger was investigated, animals were injected with, respectively, furosemide and amiloride 10 min before PGE2. Superficial and crypt colonocytes were then isolated and the protein expression of the Na+-K+ ATPase and the Na+K+2Cl- was determined by western blot analysis. The effect of PGE2 on the pump activity in presence or absence of the transporters' inhibitors was also studied. PGE2 decreased net water and chloride absorption from the colon, increased the Na+-K+ ATPase activity in superficial cells and reduced it in crypt cells. The prostaglandin was found to stimulate secretion in superficial cells by targeting the Na+K+2Cl- symporter, and reduce absorption in crypt cells by targeting the Na+/H+ antiporter. Changes in the activity of the pump are secondary to changes in the activity of the other transporters.",
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