Persistent suppression of ocular neovascularization with intravitreal administration of AAVrh.10 coding for bevacizumab

Yanxiong Mao, Szilard Kiss, Julie L. Boyer, Neil R. Hackett, Jianping Qiu, Andrew Carbone, Jason G. Mezey, Stephen M. Kaminsky, Donald J. D'Amico, Ronald G. Crystal

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Abstract

Vascular endothelial growth factor (VEGF) plays an important role in the pathogenesis of neovascular age-related macular degeneration and diabetic retinopathy. Bevacizumab, an anti-VEGF monoclonal antibody, is efficacious for these disorders, but requires monthly intravitreal administration, with associated discomfort, cost, and adverse event risk. We hypothesized that a single intravitreal administration of adeno-associated virus (AAV) vector expressing bevacizumab would result in persistent eye expression of bevacizumab and suppress VEGF-induced retinal neovascularization. We constructed an AAV rhesus serotype rh.10 vector to deliver bevacizumab (AAVrh.10BevMab) and assessed its ability to suppress neovascularization in transgenic mice overexpressing human VEGF165 in photoreceptors. Intravitreal AAVrh.10BevMab directed long-term bevacizumab expression in the retinal pigmented epithelium. Treated homozygous mice had reduced levels of neovascularization, with 90±4% reduction 168 days following treatment. Thus, a single administration of AAVrh.10BevMab provides long-term suppression of neovascularization without the costs and risks associated with the multiple administrations required for the current conventional bevacizumab monoclonal drug delivery.

Original languageEnglish
Pages (from-to)1525-1535
Number of pages11
JournalHuman gene therapy
Volume22
Issue number12
DOIs
Publication statusPublished - 1 Dec 2011

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ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Mao, Y., Kiss, S., Boyer, J. L., Hackett, N. R., Qiu, J., Carbone, A., Mezey, J. G., Kaminsky, S. M., D'Amico, D. J., & Crystal, R. G. (2011). Persistent suppression of ocular neovascularization with intravitreal administration of AAVrh.10 coding for bevacizumab. Human gene therapy, 22(12), 1525-1535. https://doi.org/10.1089/hum.2011.090