Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer

Ena Wang, Yvonne Ngalame, Monica C. Panelli, Hoainam Nguyen-Jackson, Michael Deavers, Peter Mueller, Wei Hu, Cherylyn A. Savary, Ryuji Kobayashi, Ralph S. Freedman, Francesco M. Marincola

Research output: Contribution to journalArticle

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Abstract

Purpose: Epithelial ovarian cancer (EOC) is characterized by early peritoneal involvement ultimately contributing to morbidity and mortality. To study the role of the peritoneum in fostering tumor invasion, we analyzed differences between the transcriptioaal repertoires of peritoneal tissue lacking detectable cancer in patients with EOC versus benign gynecologic disease. Experimental Design: Specimens were collected at laparotomy from patients with benign disease (b) or malignant (m) ovarian pathology and comprised primary ovarian tumors, paired bilateral specimens from adjacent peritoneum and attached stroma (PE), subjacent stroma (ST), peritoneal washes, ascites, and peripheral blood mononuclear cells. Specimens were immediately frozen. RNA was amplified by in vitro transcription and cohybridized with reference RNA to a custom-made 17.5k cDNA microarray. Results: Principal component analysis and unsupervised clustering did not segregate specimens from patients with benign or malignant pathology. Class comparison identified differences between benign and malignant PE and ST specimens deemed significant by permutation test (P = 0.027 and 0.012, respectively). A two-tailed Student's t test identified 402 (bPE versus mPE) and 663 (mST versus bST) genes differentially expressed at a significance level of P2 ≤ 0.005 when all available paired samples from each patient were analyzed. The same comparison using one sample per patient reduced the pool of differentially expressed genes but retained permutation test significance for bST versus mST (P = 0.031) and borderline significance for bPE versus mPE (P = 0.056) differences. Conclusions: The presence of EOC may foster peritoneal implantation and growth of cancer cells by inducing factors that may represent molecular targets for disease control.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalClinical Cancer Research
Volume11
Issue number1
Publication statusPublished - 1 Jan 2005
Externally publishedYes

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Ovarian Neoplasms
Peritoneum
Neoplasms
Female Genital Diseases
RNA
Pathology
Foster Home Care
Principal Component Analysis
Oligonucleotide Array Sequence Analysis
Ascites
Laparotomy
Genes
Cluster Analysis
Blood Cells
Research Design
Students
Morbidity
Mortality
Growth
Ovarian epithelial cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wang, E., Ngalame, Y., Panelli, M. C., Nguyen-Jackson, H., Deavers, M., Mueller, P., ... Marincola, F. M. (2005). Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer. Clinical Cancer Research, 11(1), 113-122.

Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer. / Wang, Ena; Ngalame, Yvonne; Panelli, Monica C.; Nguyen-Jackson, Hoainam; Deavers, Michael; Mueller, Peter; Hu, Wei; Savary, Cherylyn A.; Kobayashi, Ryuji; Freedman, Ralph S.; Marincola, Francesco M.

In: Clinical Cancer Research, Vol. 11, No. 1, 01.01.2005, p. 113-122.

Research output: Contribution to journalArticle

Wang, E, Ngalame, Y, Panelli, MC, Nguyen-Jackson, H, Deavers, M, Mueller, P, Hu, W, Savary, CA, Kobayashi, R, Freedman, RS & Marincola, FM 2005, 'Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer', Clinical Cancer Research, vol. 11, no. 1, pp. 113-122.
Wang E, Ngalame Y, Panelli MC, Nguyen-Jackson H, Deavers M, Mueller P et al. Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer. Clinical Cancer Research. 2005 Jan 1;11(1):113-122.
Wang, Ena ; Ngalame, Yvonne ; Panelli, Monica C. ; Nguyen-Jackson, Hoainam ; Deavers, Michael ; Mueller, Peter ; Hu, Wei ; Savary, Cherylyn A. ; Kobayashi, Ryuji ; Freedman, Ralph S. ; Marincola, Francesco M. / Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer. In: Clinical Cancer Research. 2005 ; Vol. 11, No. 1. pp. 113-122.
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AU - Mueller, Peter

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N2 - Purpose: Epithelial ovarian cancer (EOC) is characterized by early peritoneal involvement ultimately contributing to morbidity and mortality. To study the role of the peritoneum in fostering tumor invasion, we analyzed differences between the transcriptioaal repertoires of peritoneal tissue lacking detectable cancer in patients with EOC versus benign gynecologic disease. Experimental Design: Specimens were collected at laparotomy from patients with benign disease (b) or malignant (m) ovarian pathology and comprised primary ovarian tumors, paired bilateral specimens from adjacent peritoneum and attached stroma (PE), subjacent stroma (ST), peritoneal washes, ascites, and peripheral blood mononuclear cells. Specimens were immediately frozen. RNA was amplified by in vitro transcription and cohybridized with reference RNA to a custom-made 17.5k cDNA microarray. Results: Principal component analysis and unsupervised clustering did not segregate specimens from patients with benign or malignant pathology. Class comparison identified differences between benign and malignant PE and ST specimens deemed significant by permutation test (P = 0.027 and 0.012, respectively). A two-tailed Student's t test identified 402 (bPE versus mPE) and 663 (mST versus bST) genes differentially expressed at a significance level of P2 ≤ 0.005 when all available paired samples from each patient were analyzed. The same comparison using one sample per patient reduced the pool of differentially expressed genes but retained permutation test significance for bST versus mST (P = 0.031) and borderline significance for bPE versus mPE (P = 0.056) differences. Conclusions: The presence of EOC may foster peritoneal implantation and growth of cancer cells by inducing factors that may represent molecular targets for disease control.

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