Pbcrk-1, the Plasmodium berghei orthologue of P. falciparum cdc-2 related kinase-1 (Pfcrk-1), is essential for completion of the intraerythrocytic asexual cycle

Radha Rangarajan, Amy Bei, Natohya Henry, Marylin Madamet, Daniel Parzy, Marie Paule Nivez, Christian Doerig, Ali Sultan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The molecular mechanisms underlying gametocytogenesis in malaria parasites are not understood. Plasmodium falciparum cdc2-related kinase 1 (pfcrk-1), a gene that is expressed predominantly in gametocytes, bears homology to the PITSLRE subfamily of cyclin-dependent kinases and has been hypothesized to function as a negative regulator of the cell cycle. We attempted to knock-out pbcrk-1, the P. berghei orthologue of pfcrk-1, but were unable to recover P. berghei parasites with a disrupted pbcrk-1 locus. In contrast, an integration event at this locus that did not result in a loss-of-function of the pbcrk-1 gene was readily observed. This strongly suggests that a functional pbcrk-1 gene product is essential to intraerythrocytic asexual multiplication.

Original languageEnglish
Pages (from-to)202-207
Number of pages6
JournalExperimental Parasitology
Volume112
Issue number3
DOIs
Publication statusPublished - Mar 2006

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Plasmodium berghei
Phosphotransferases
Plasmodium falciparum
Parasites
Genes
Cyclin-Dependent Kinases
Malaria
Cell Cycle

Keywords

  • Cell cycle
  • Phosphorylation
  • Plasmodium
  • Protein kinase
  • Reverse genetics
  • Signal transduction

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Pbcrk-1, the Plasmodium berghei orthologue of P. falciparum cdc-2 related kinase-1 (Pfcrk-1), is essential for completion of the intraerythrocytic asexual cycle. / Rangarajan, Radha; Bei, Amy; Henry, Natohya; Madamet, Marylin; Parzy, Daniel; Nivez, Marie Paule; Doerig, Christian; Sultan, Ali.

In: Experimental Parasitology, Vol. 112, No. 3, 03.2006, p. 202-207.

Research output: Contribution to journalArticle

Rangarajan, Radha ; Bei, Amy ; Henry, Natohya ; Madamet, Marylin ; Parzy, Daniel ; Nivez, Marie Paule ; Doerig, Christian ; Sultan, Ali. / Pbcrk-1, the Plasmodium berghei orthologue of P. falciparum cdc-2 related kinase-1 (Pfcrk-1), is essential for completion of the intraerythrocytic asexual cycle. In: Experimental Parasitology. 2006 ; Vol. 112, No. 3. pp. 202-207.
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