Serine proteases can induce cell signaling by stimulating G-protein-coupled receptors, called proteinase-activated receptors (PAR's) on a variety of epithelial cells. While PAR-2, one such receptor, activates cell signaling in a secretory cell line derived from human sweat glands, there was no information on their presence and effects on intact sweat glands. PAR-2 presence and activation of eccrine sweat glands isolated from human skin samples was investigated using Western blot analysis, immunohistochemistry, electron microscopy (EM) and Ca2+ imaging. Anti-human PAR-2 antibody demonstrated the presence of these receptors in eccrine sweat glands. EM showed that PAR-2 activation resulted in degranulation of secretory cells. Ca 2+ imaging using PAR-2 activators demonstrated a two phase increase in [Ca2+]i which was dependent on extracellular Ca 2+ for the second phase, and that the response could be blocked by prior incubation with xestospongin, the IP3 receptor blocker. The results demonstrated that PAR-2 receptors are present in human sweat gland secretory cells and that these receptors are functionally active and can induce changes associated with secretory events in eccrine glands.
- Eccrine sweat glands
- Proteinase activated receptors
- Ussing chamber
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)