Paper electrophoresis and chromatography of oligopeptides with N-terminal methionine

Norton A. Elson, Ronald Crystal, W. French Anderson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Several electrophoretic and chromatographic systems are described for the separation of di- and tripeptides with N-terminal methionine. The most effective methods of separation are electrophoresis in barbital-triethylamine-acetic acid, pH 8.0 and chromatography in n-butanol-acetic acid-water, 18:2:5. Increased resolution may be obtained by using two or more systems in combination. These methods were intended to separate and identify the oligopeptides formed during the initiation of eukaryotic protein synthesis, but may also be applied to the separation of small peptides generally.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalAnalytical Biochemistry
Volume54
Issue number1
DOIs
Publication statusPublished - 1 Jan 1973
Externally publishedYes

Fingerprint

Paper Electrophoresis
Paper Chromatography
Oligopeptides
Chromatography
Electrophoresis
Acetic Acid
Methionine
Barbital
1-Butanol
Dipeptides
Peptides
Water
Proteins
triethylamine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Paper electrophoresis and chromatography of oligopeptides with N-terminal methionine. / Elson, Norton A.; Crystal, Ronald; Anderson, W. French.

In: Analytical Biochemistry, Vol. 54, No. 1, 01.01.1973, p. 161-169.

Research output: Contribution to journalArticle

Elson, Norton A. ; Crystal, Ronald ; Anderson, W. French. / Paper electrophoresis and chromatography of oligopeptides with N-terminal methionine. In: Analytical Biochemistry. 1973 ; Vol. 54, No. 1. pp. 161-169.
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