Paper electrophoresis and chromatography of oligopeptides with N-terminal methionine

Norton A. Elson, Ronald G. Crystal, W. French Anderson

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Several electrophoretic and chromatographic systems are described for the separation of di- and tripeptides with N-terminal methionine. The most effective methods of separation are electrophoresis in barbital-triethylamine-acetic acid, pH 8.0 and chromatography in n-butanol-acetic acid-water, 18:2:5. Increased resolution may be obtained by using two or more systems in combination. These methods were intended to separate and identify the oligopeptides formed during the initiation of eukaryotic protein synthesis, but may also be applied to the separation of small peptides generally.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - Jul 1973


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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