Palette of fluorinated voltage-sensitive hemicyanine dyes

Ping Yan, Corey D. Acker, Wen Liang Zhou, Peter Lee, Christian Bollensdorff, Adrian Negreane, Jacopo Lotti, Leonardo Sacconi, Srdjan D. Antic, Peter Kohl, Huibert D. Mansvelder, Francesco S. Pavone, Leslie M. Loew

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Optical recording of membrane potential permits spatially resolved measurement of electrical activity in subcellular regions of single cells, which would be inaccessible to electrodes, and imaging of spatiotemporal patterns of action potential propagation in excitable tissues, such as the brain or heart. However, the available voltage-sensitive dyes (VSDs) are not always spectrally compatible with newly available optical technologies for sensing or manipulating the physiological state of a system. Here, we describe a series of 19 fluorinated VSDs based on the hemicyanine class of chromophores. Strategic placement of the fluorine atoms on the chromophores can result in either blue or red shifts in the absorbance and emission spectra. The range of one-photon excitation wavelengths afforded by these new VSDs spans 440-670 nm; the twophoton excitation range is 900-1,340 nm. The emission of each VSD is shifted by at least 100 nm to the red of its one-photon excitation spectrum. The set of VSDs, thus, affords an extended toolkit for optical recording to match a broad range of experimental requirements. We show the sensitivity to voltage and the photostability of the new VSDs in a series of experimental preparations ranging in scale from single dendritic spines to whole heart. Among the advances shown in these applications are simultaneous recording of voltage and calcium in single dendritic spines and optical electrophysiology recordings using two-photon excitation above 1,100 nm.

Original languageEnglish
Pages (from-to)20443-20448
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number50
DOIs
Publication statusPublished - 11 Dec 2012
Externally publishedYes

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Coloring Agents
Photons
Dendritic Spines
Fluorine
Electrophysiology
Membrane Potentials
Action Potentials
tetramethylene hemicyanine
Electrodes
Calcium
Technology
Brain

Keywords

  • Fluorescence
  • Microscopy

ASJC Scopus subject areas

  • General

Cite this

Yan, P., Acker, C. D., Zhou, W. L., Lee, P., Bollensdorff, C., Negreane, A., ... Loew, L. M. (2012). Palette of fluorinated voltage-sensitive hemicyanine dyes. Proceedings of the National Academy of Sciences of the United States of America, 109(50), 20443-20448. https://doi.org/10.1073/pnas.1214850109

Palette of fluorinated voltage-sensitive hemicyanine dyes. / Yan, Ping; Acker, Corey D.; Zhou, Wen Liang; Lee, Peter; Bollensdorff, Christian; Negreane, Adrian; Lotti, Jacopo; Sacconi, Leonardo; Antic, Srdjan D.; Kohl, Peter; Mansvelder, Huibert D.; Pavone, Francesco S.; Loew, Leslie M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 50, 11.12.2012, p. 20443-20448.

Research output: Contribution to journalArticle

Yan, P, Acker, CD, Zhou, WL, Lee, P, Bollensdorff, C, Negreane, A, Lotti, J, Sacconi, L, Antic, SD, Kohl, P, Mansvelder, HD, Pavone, FS & Loew, LM 2012, 'Palette of fluorinated voltage-sensitive hemicyanine dyes', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 50, pp. 20443-20448. https://doi.org/10.1073/pnas.1214850109
Yan, Ping ; Acker, Corey D. ; Zhou, Wen Liang ; Lee, Peter ; Bollensdorff, Christian ; Negreane, Adrian ; Lotti, Jacopo ; Sacconi, Leonardo ; Antic, Srdjan D. ; Kohl, Peter ; Mansvelder, Huibert D. ; Pavone, Francesco S. ; Loew, Leslie M. / Palette of fluorinated voltage-sensitive hemicyanine dyes. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 50. pp. 20443-20448.
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