Oxidants spontaneously released by alveolar macrophages of cigarette smokers can inactivate the active site of α1-antitrypsin, rendering it ineffective as an inhibitor of neutrophil elastase

R. C. Hubbard, F. Ogushi, G. A. Fells, A. M. Cantin, S. Jallat, M. Courtney, R. G. Crystal

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Abstract

Current concepts relating to the pathogenesis of emphysema associated with cigarette smoking is that an imbalance exists within the lower respiratory tract between neutrophil elastase and the local anti-neutrophil elastase screen, enabling uninhibited neutrophil elastase to destroy the alveolar structures over time. The possible role of alveolar macrophages in contributing to this imbalance was investigated by evaluating the ability of cigarette smokers' alveolar macrophages to inactivate alpha 1-antitrypsin (α1AT), the major anti-neutrophil elastase of the human lower respiratory tract. In vitro, alveolar macrophages of smokers spontaneously released 2.5-fold more superoxide anion and eightfold more H2O2 than macrophages of nonsmokers (P < 0.01, both comparisons). Using a model system that reproduced the relative amounts of alveolar macrophages and α1AT found in the epithelial lining fluid of the lower respiratory tract, we observed that smokers' macrophages caused a 60 ± 5% reduction in the ability of α1AT to inhibit neutrophil elastase. In marked contrast, under the same conditions, nonsmokers' macrophages had no effect upon the anti-neutrophil elastase function of α1AT. Addition of superoxide dismutase, catalase, mannitol, and methionine prevented inactivation of α1AT by smokers' macrophages, implying that the release of oxidants mediated the inactivation of α1AT. In addition, by utilizing a recombinant DNA produced modified form of α1AT containing an active site substitution (met358 → val), the inactivation of α1AT by smokers' alveolar macrophages was prevented, suggesting that the smokers' macrophages inactivate α1AT by oxidizing the active site of the α1AT molecule. These results suggest that in cigarette smokers, the alveolar macrophage can modulate the activity of α1AT as an inhibitor of neutrophil elastase and thus play a role in the pathogenesis of emphysema associated with cigarette smoking.

Original languageEnglish
Pages (from-to)1289-1295
Number of pages7
JournalJournal of Clinical Investigation
Volume80
Issue number5
DOIs
Publication statusPublished - 1 Jan 1987

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ASJC Scopus subject areas

  • Medicine(all)

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