Overexpression of inducible 70-kDa heat shock protein in mouse improves structural and functional recovery of skeletal muscles from atrophy

Elen H. Miyabara, Tabata L. Nascimento, Débora C. Rodrigues, Anselmo S. Moriscot, Wilmer F. Davila, Younss AitMou, Pieter P. DeTombe, Ruben Mestril

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Heat shock proteins play a key regulatory role in cellular defense. To investigate the role of the inducible 70-kDa heat shock protein (HSP70) in skeletal muscle atrophy and subsequent recovery, soleus (SOL) and extensor digitorum longus (EDL) muscles from overexpressing HSP70 transgenic mice were immobilized for 7 days and subsequently released from immobilization and evaluated after 7 days. Histological analysis showed that there was a decrease in cross-sectional area of type II myofiber from EDL and types I and II myofiber from SOL muscles at 7-day immobilization in both wild-type and HSP70 mice. At 7-day recovery, EDL and SOL myofibers from HSP70 mice, but not from wild-type mice, recovered their size. Muscle tetanic contraction decreased only in SOL muscles fromwild-type mice at both 7-day immobilization and 7-day recovery; however, it was unaltered in the respective groups from HSP70 mice. Although no effect in a fatigue protocol was observed among groups, we noticed a better contractile performance of EDL muscles from overexpressing HSP70 groups as compared to their matched wild-type groups. The number of NCAM positive-satellite cells reduced after immobilization and recovery in both EDL and SOL muscles fromwild-typemice, but it was unchanged in themuscles from HSP70 mice. These results suggest that HSP70 improves structural and functional recovery of skeletal muscle after disuse atrophy, and this effect might be associated with preservation of satellite cell amount.

Original languageEnglish
Pages (from-to)733-741
Number of pages9
JournalPflugers Archiv European Journal of Physiology
Volume463
Issue number5
DOIs
Publication statusPublished - May 2012
Externally publishedYes

Fingerprint

HSP70 Heat-Shock Proteins
Muscular Atrophy
Muscle
Skeletal Muscle
Recovery
Immobilization
Atrophic Muscular Disorders
Neural Cell Adhesion Molecules
Muscles
Satellites
Muscle Contraction
Heat-Shock Proteins
Transgenic Mice
Fatigue
Research Design
Fatigue of materials

Keywords

  • Force production
  • Heat shock protein
  • Muscle atrophy
  • Myofiber mass recovery
  • Transgenic mouse

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Overexpression of inducible 70-kDa heat shock protein in mouse improves structural and functional recovery of skeletal muscles from atrophy. / Miyabara, Elen H.; Nascimento, Tabata L.; Rodrigues, Débora C.; Moriscot, Anselmo S.; Davila, Wilmer F.; AitMou, Younss; DeTombe, Pieter P.; Mestril, Ruben.

In: Pflugers Archiv European Journal of Physiology, Vol. 463, No. 5, 05.2012, p. 733-741.

Research output: Contribution to journalArticle

Miyabara, Elen H. ; Nascimento, Tabata L. ; Rodrigues, Débora C. ; Moriscot, Anselmo S. ; Davila, Wilmer F. ; AitMou, Younss ; DeTombe, Pieter P. ; Mestril, Ruben. / Overexpression of inducible 70-kDa heat shock protein in mouse improves structural and functional recovery of skeletal muscles from atrophy. In: Pflugers Archiv European Journal of Physiology. 2012 ; Vol. 463, No. 5. pp. 733-741.
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