Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements

Caitlin Howell, Hicham Hamoudi, Stefan Heissler, Patrick Koelsch, Michael Zharnikov

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4 Citations (Scopus)

Abstract

Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalChemical Physics Letters
Volume513
Issue number4-6
DOIs
Publication statusPublished - 15 Sep 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

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