Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements

Caitlin Howell, Hicham Hamoudi, Stefan Heissler, Patrick Koelsch, Michael Zharnikov

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalChemical Physics Letters
Volume513
Issue number4-6
DOIs
Publication statusPublished - 15 Sep 2011
Externally publishedYes

Fingerprint

deoxyribonucleic acid
preparation
DNA
X ray absorption near edge structure spectroscopy
Spectroscopic analysis
Water
oligonucleotides
infrared reflection
spectroscopic analysis
Absorption spectroscopy
Oligonucleotides
Gold
water
absorption spectroscopy
x rays
X ray photoelectron spectroscopy
fine structure
photoelectron spectroscopy
gold
Infrared radiation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

Cite this

Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements. / Howell, Caitlin; Hamoudi, Hicham; Heissler, Stefan; Koelsch, Patrick; Zharnikov, Michael.

In: Chemical Physics Letters, Vol. 513, No. 4-6, 15.09.2011, p. 267-270.

Research output: Contribution to journalArticle

Howell, Caitlin ; Hamoudi, Hicham ; Heissler, Stefan ; Koelsch, Patrick ; Zharnikov, Michael. / Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements. In: Chemical Physics Letters. 2011 ; Vol. 513, No. 4-6. pp. 267-270.
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