Orexin A immunoreactivity and preproorexin mRNA in the brain of Zucker and WKY rats

Shahrad Taheri, J. Gardiner, S. Hafizi, K. Murphy, C. Dakin, L. Seal, C. Small, M. Ghatei, S. Bloom

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The primary role of the orexins was originally believed to be appetite regulation, but is now believed to be the regulation of sleep, arousal and locomotor activity. Orexin A immunoreactivity (orexin A-IR) and prepro-orexin mRNA were measured in the CNS of obese and lean Zucker rats. There were no differences in orexin A-IR or prepro-orexin mRNA levels between obese and lean Zucker rats. The orexins are therefore unlikely to be important in this model of obesity. Levels of orexin A-IR and prepro-orexin mRNA were measured in the CNS of Wistar-Kyoto (WKY) rats, which are hypoactive and have abnormal sleep architecture. Compared to Wistar rats, WKY rats had significantly lower orexin A-IR (with differences of up to 100% in some brain regions) and prepro-orexin mRNA levels. These observations suggest that the sleep and activity phenotype of the WKY strain may be related to orexin deficiency and that this strain may be a useful model of partial orexin deficiency.

Original languageEnglish
Pages (from-to)459-464
Number of pages6
JournalNeuroReport
Volume12
Issue number3
Publication statusPublished - 5 Mar 2001
Externally publishedYes

Fingerprint

Inbred WKY Rats
Messenger RNA
Brain
Zucker Rats
Sleep
Appetite Regulation
Locomotion
Arousal
Orexins
Wistar Rats
Obesity
Phenotype

Keywords

  • Hypocretins
  • Locomotor activity
  • Obesity
  • Orexin A immunoreactivity
  • Orexins
  • Prepro-orexin mRNA
  • Sleep
  • WKY rats
  • Zucker rats

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Taheri, S., Gardiner, J., Hafizi, S., Murphy, K., Dakin, C., Seal, L., ... Bloom, S. (2001). Orexin A immunoreactivity and preproorexin mRNA in the brain of Zucker and WKY rats. NeuroReport, 12(3), 459-464.

Orexin A immunoreactivity and preproorexin mRNA in the brain of Zucker and WKY rats. / Taheri, Shahrad; Gardiner, J.; Hafizi, S.; Murphy, K.; Dakin, C.; Seal, L.; Small, C.; Ghatei, M.; Bloom, S.

In: NeuroReport, Vol. 12, No. 3, 05.03.2001, p. 459-464.

Research output: Contribution to journalArticle

Taheri, S, Gardiner, J, Hafizi, S, Murphy, K, Dakin, C, Seal, L, Small, C, Ghatei, M & Bloom, S 2001, 'Orexin A immunoreactivity and preproorexin mRNA in the brain of Zucker and WKY rats', NeuroReport, vol. 12, no. 3, pp. 459-464.
Taheri S, Gardiner J, Hafizi S, Murphy K, Dakin C, Seal L et al. Orexin A immunoreactivity and preproorexin mRNA in the brain of Zucker and WKY rats. NeuroReport. 2001 Mar 5;12(3):459-464.
Taheri, Shahrad ; Gardiner, J. ; Hafizi, S. ; Murphy, K. ; Dakin, C. ; Seal, L. ; Small, C. ; Ghatei, M. ; Bloom, S. / Orexin A immunoreactivity and preproorexin mRNA in the brain of Zucker and WKY rats. In: NeuroReport. 2001 ; Vol. 12, No. 3. pp. 459-464.
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