Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model

Peter Kubatka, Martin Kello, Karol Kajo, Peter Kruzliak, Desanka Výbohová, Ján Mojžiš, Marián Adamkov, Silvia Fialová, Lucia Veizerová, Anthony Zulli, Martin Péč, Dagmar Statelová, Daniel Grančai, Dietrich Busselberg

Research output: Contribution to journalArticle

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Abstract

Purpose: There has been a considerable interest in the identification of natural plant foods for developing effective agents against cancer. Thus, the anti-tumour effects of oregano in the in vivo and in vitro breast cancer model were evaluated. Methods: Lyophilized oregano (ORE) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration. At autopsy, mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation in MCF-7 cells was carried out. Results: Low-dose ORE suppressed tumour frequency by 55.5 %, tumour incidence by 44 %, and tumour volume by 44.5 % compared to control animals. Analysis of rat tumour cells showed Ki67, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase after low-dose ORE treatment. High-dose ORE lengthened tumour latency by 12.5 days; moreover, Bcl-2, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase in carcinoma cells were observed. Histopathological analysis revealed a decrease in the ratio of high-/low-grade carcinomas in both treated groups. In vitro studies showed that ORE decreased survival and proliferation of MCF-7 cells. In ORE-treated MCF-7 cells, an increase in cells expressing sub-G0/G1 DNA content and an increase in the percentage of annexin V/PI positive MCF-7 cells were observed. In vitro, both caspase-dependent and possible non-caspase-dependent apoptotic pathways were found. The deactivation of anti-apoptotic activity of Bcl-2, a decrease in mitochondrial membrane potential, and the activation of mitochondrial apoptosis pathway were observed in the ORE-treated MCF-7 cells. Conclusions: Our results demonstrate, for the first time, a distinct tumour-suppressive effect of oregano in the breast cancer model.

Original languageEnglish
Pages (from-to)1303-1316
Number of pages14
JournalEuropean Journal of Nutrition
Volume56
Issue number3
DOIs
Publication statusPublished - 1 Apr 2017

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Origanum
Breast Neoplasms
MCF-7 Cells
Neoplasms
Vascular Endothelial Growth Factor Receptor-2
Caspase 3
Carcinoma
Edible Plants
Mitochondrial Membrane Potential
Annexin A5
Caspases
Tumor Burden
Carcinogens
Autopsy
Apoptosis
Diet

Keywords

  • Angiogenesis
  • Apoptosis
  • Cancer stem cells
  • Cell proliferation
  • Mammary carcinogenesis
  • MCF-7 cells
  • Oregano
  • Rat

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model. / Kubatka, Peter; Kello, Martin; Kajo, Karol; Kruzliak, Peter; Výbohová, Desanka; Mojžiš, Ján; Adamkov, Marián; Fialová, Silvia; Veizerová, Lucia; Zulli, Anthony; Péč, Martin; Statelová, Dagmar; Grančai, Daniel; Busselberg, Dietrich.

In: European Journal of Nutrition, Vol. 56, No. 3, 01.04.2017, p. 1303-1316.

Research output: Contribution to journalArticle

Kubatka, P, Kello, M, Kajo, K, Kruzliak, P, Výbohová, D, Mojžiš, J, Adamkov, M, Fialová, S, Veizerová, L, Zulli, A, Péč, M, Statelová, D, Grančai, D & Busselberg, D 2017, 'Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model', European Journal of Nutrition, vol. 56, no. 3, pp. 1303-1316. https://doi.org/10.1007/s00394-016-1181-5
Kubatka P, Kello M, Kajo K, Kruzliak P, Výbohová D, Mojžiš J et al. Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model. European Journal of Nutrition. 2017 Apr 1;56(3):1303-1316. https://doi.org/10.1007/s00394-016-1181-5
Kubatka, Peter ; Kello, Martin ; Kajo, Karol ; Kruzliak, Peter ; Výbohová, Desanka ; Mojžiš, Ján ; Adamkov, Marián ; Fialová, Silvia ; Veizerová, Lucia ; Zulli, Anthony ; Péč, Martin ; Statelová, Dagmar ; Grančai, Daniel ; Busselberg, Dietrich. / Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model. In: European Journal of Nutrition. 2017 ; Vol. 56, No. 3. pp. 1303-1316.
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AU - Kello, Martin

AU - Kajo, Karol

AU - Kruzliak, Peter

AU - Výbohová, Desanka

AU - Mojžiš, Ján

AU - Adamkov, Marián

AU - Fialová, Silvia

AU - Veizerová, Lucia

AU - Zulli, Anthony

AU - Péč, Martin

AU - Statelová, Dagmar

AU - Grančai, Daniel

AU - Busselberg, Dietrich

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N2 - Purpose: There has been a considerable interest in the identification of natural plant foods for developing effective agents against cancer. Thus, the anti-tumour effects of oregano in the in vivo and in vitro breast cancer model were evaluated. Methods: Lyophilized oregano (ORE) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration. At autopsy, mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation in MCF-7 cells was carried out. Results: Low-dose ORE suppressed tumour frequency by 55.5 %, tumour incidence by 44 %, and tumour volume by 44.5 % compared to control animals. Analysis of rat tumour cells showed Ki67, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase after low-dose ORE treatment. High-dose ORE lengthened tumour latency by 12.5 days; moreover, Bcl-2, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase in carcinoma cells were observed. Histopathological analysis revealed a decrease in the ratio of high-/low-grade carcinomas in both treated groups. In vitro studies showed that ORE decreased survival and proliferation of MCF-7 cells. In ORE-treated MCF-7 cells, an increase in cells expressing sub-G0/G1 DNA content and an increase in the percentage of annexin V/PI positive MCF-7 cells were observed. In vitro, both caspase-dependent and possible non-caspase-dependent apoptotic pathways were found. The deactivation of anti-apoptotic activity of Bcl-2, a decrease in mitochondrial membrane potential, and the activation of mitochondrial apoptosis pathway were observed in the ORE-treated MCF-7 cells. Conclusions: Our results demonstrate, for the first time, a distinct tumour-suppressive effect of oregano in the breast cancer model.

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KW - Mammary carcinogenesis

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KW - Oregano

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