Orai channels are critical for receptor-mediated endocytosis of albumin

Bo Zeng, Gui Lan Chen, Eliana Garcia-Vaz, Sunil Bhandari, Nikoleta Daskoulidou, Lisa M. Berglund, Hongni Jiang, Thomas Hallett, Lu Ping Zhou, Li Huang, Zi Hao Xu, Viji Nair, Robert G. Nelson, Wenjun Ju, Matthias Kretzler, Stephen Atkin, Maria F. Gomez, Shang Zhong Xu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Impaired albumin reabsorption by proximal tubular epithelial cells (PTECs) has been highlighted in diabetic nephropathy (DN), but little is known about the underlying molecular mechanisms. Here we find that ORAI1-3, are preferentially expressed in PTECs and downregulated in patients with DN. Hyperglycemia or blockade of insulin signaling reduces the expression of ORAI1-3. Inhibition of ORAI channels by BTP2 and diethylstilbestrol or silencing of ORAI expression impairs albumin uptake. Transgenic mice expressing a dominant-negative Orai1 mutant (E108Q) increases albuminuria, and in vivo injection of BTP2 exacerbates albuminuria in streptozotocin-induced and Akita diabetic mice. The albumin endocytosis is Ca2+-dependent and accompanied by ORAI1 internalization. Amnionless (AMN) associates with ORAIs and forms STIM/ORAI/AMN complexes after Ca2+ store depletion. STIM1/ORAI1 colocalizes with clathrin, but not with caveolin, at the apical membrane of PTECs, which determines clathrin-mediated endocytosis. These findings provide insights into the mechanisms of protein reabsorption and potential targets for treating diabetic proteinuria.

Original languageEnglish
Article number1920
JournalNature Communications
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Endocytosis
albumins
Albumins
Clathrin
Albuminuria
Epithelial Cells
Diabetic Nephropathies
mice
hyperglycemia
Caveolins
insulin
Diethylstilbestrol
Streptozocin
Proteinuria
Hyperglycemia
Transgenic Mice
depletion
Down-Regulation
Insulin
injection

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Zeng, B., Chen, G. L., Garcia-Vaz, E., Bhandari, S., Daskoulidou, N., Berglund, L. M., ... Xu, S. Z. (2017). Orai channels are critical for receptor-mediated endocytosis of albumin. Nature Communications, 8(1), [1920]. https://doi.org/10.1038/s41467-017-02094-y

Orai channels are critical for receptor-mediated endocytosis of albumin. / Zeng, Bo; Chen, Gui Lan; Garcia-Vaz, Eliana; Bhandari, Sunil; Daskoulidou, Nikoleta; Berglund, Lisa M.; Jiang, Hongni; Hallett, Thomas; Zhou, Lu Ping; Huang, Li; Xu, Zi Hao; Nair, Viji; Nelson, Robert G.; Ju, Wenjun; Kretzler, Matthias; Atkin, Stephen; Gomez, Maria F.; Xu, Shang Zhong.

In: Nature Communications, Vol. 8, No. 1, 1920, 01.12.2017.

Research output: Contribution to journalArticle

Zeng, B, Chen, GL, Garcia-Vaz, E, Bhandari, S, Daskoulidou, N, Berglund, LM, Jiang, H, Hallett, T, Zhou, LP, Huang, L, Xu, ZH, Nair, V, Nelson, RG, Ju, W, Kretzler, M, Atkin, S, Gomez, MF & Xu, SZ 2017, 'Orai channels are critical for receptor-mediated endocytosis of albumin', Nature Communications, vol. 8, no. 1, 1920. https://doi.org/10.1038/s41467-017-02094-y
Zeng B, Chen GL, Garcia-Vaz E, Bhandari S, Daskoulidou N, Berglund LM et al. Orai channels are critical for receptor-mediated endocytosis of albumin. Nature Communications. 2017 Dec 1;8(1). 1920. https://doi.org/10.1038/s41467-017-02094-y
Zeng, Bo ; Chen, Gui Lan ; Garcia-Vaz, Eliana ; Bhandari, Sunil ; Daskoulidou, Nikoleta ; Berglund, Lisa M. ; Jiang, Hongni ; Hallett, Thomas ; Zhou, Lu Ping ; Huang, Li ; Xu, Zi Hao ; Nair, Viji ; Nelson, Robert G. ; Ju, Wenjun ; Kretzler, Matthias ; Atkin, Stephen ; Gomez, Maria F. ; Xu, Shang Zhong. / Orai channels are critical for receptor-mediated endocytosis of albumin. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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