"One cell-one well"

A new approach to inkjet printing single cell microarrays

Albert R. Liberski, Joseph T. Delaney, Ulrich S. Schubert

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A new approach to prepare arrays of sessile droplets of living single cell cultures using a liquid hydrophobic barrier prevents the samples from dehydrating, and allows for spatially addressable arrays for statistical quantitative single cell studies. By carefully advancing a thin layer ofmineral oil on the substrate over the droplets during the printing, dehydration of the droplets can be prevented, and the vitality of the cells can be maintained. The net result of this confluence of submerged cell culturing and inkjet printing is facile access to spatially addressable arrays of isolated single cells on surfaces. Such single cell arrays may be particularly useful as high-throughput tools in the rapidly emerging "omics" fields of cell biology.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalACS Combinatorial Science
Volume13
Issue number2
DOIs
Publication statusPublished - 14 Mar 2011
Externally publishedYes

Fingerprint

Microarrays
Printing
Cytology
Dehydration
Cell culture
Oils
Throughput
Liquids
Substrates

Keywords

  • Inkjet printing
  • Microdroplets
  • One cell-one well
  • Single cell microarrays

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

"One cell-one well" : A new approach to inkjet printing single cell microarrays. / Liberski, Albert R.; Delaney, Joseph T.; Schubert, Ulrich S.

In: ACS Combinatorial Science, Vol. 13, No. 2, 14.03.2011, p. 190-195.

Research output: Contribution to journalArticle

Liberski, Albert R. ; Delaney, Joseph T. ; Schubert, Ulrich S. / "One cell-one well" : A new approach to inkjet printing single cell microarrays. In: ACS Combinatorial Science. 2011 ; Vol. 13, No. 2. pp. 190-195.
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