Oncologic trogocytosis with Hospicells induces the expression of N-cadherin by breast cancer cells

Raphael Lis, Jérome Capdet, Pejman Mirshahi, Magali Lacroix-Triki, Francoise Dagonnet, Christophe Klein, Massoud Mirshahi, Jean Jacques Fournié, Arash Rafii Tabrizi, Mary Poupot

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In breast cancers, the appearance of metastasis is synonymous with poor prognosis. The metastatic process is usually associated with epithelial-mesenchymal transition (EMT) which is often induced by several soluble factors produced either by the tumour cells themselves or by cells constituting the tumour microenvironment. The aim of the present study was to determine whether the mesenchymal properties given by some molecules such as N-cadherin, for instance, could be acquired by cancer cells via the trogocytosis process with cells of the tumour microenvironment. Hospicells are stromal cells which were first isolated from cancer cell aggregates of patients with ovarian cancer. We recently showed that these cells are immunosuppressive for T lymphocyte functions and confer chemoresistance to cancer cells by the transfer of the MDR protein via trogocytosis. In this study, we showed that a mammary cancer cell line (MDA-MB-231) acquires patches of membrane via oncologic trogocytosis with Hospicells. This unidirectional and active process depends on actin polymerization and can be increased via inhibition of the Src family and decreased via inhibition of PI3K. Trogocytosis between Hospicells and MDA-MB-231 does not lead to the direct acquisition of N-cadherin but rather it leads to the production of soluble factor(s) which induce de novo expression of N-cadherin by the cancer cells. The novelty here is that this factor is produced only if cancer cells interact and undergo trogocytosis with Hospicells. This new expression could confer a more invasive phenotype to the cancer cells and thus can explain the correlation of the presence of Hospicells with the number of invaded lymph nodes in patients with mammary adenocarcinoma.

Original languageEnglish
Pages (from-to)1453-1461
Number of pages9
JournalInternational Journal of Oncology
Volume37
Issue number6
DOIs
Publication statusPublished - Dec 2010
Externally publishedYes

Fingerprint

Cadherins
Breast Neoplasms
Neoplasms
Tumor Microenvironment
Cellular Microenvironment
Epithelial-Mesenchymal Transition
Immunosuppressive Agents
Stromal Cells
Phosphatidylinositol 3-Kinases
Polymerization
Ovarian Neoplasms
Actins
Adenocarcinoma
Breast
Lymph Nodes
Neoplasm Metastasis
T-Lymphocytes
Phenotype
Cell Line
Membranes

Keywords

  • Epithelial-mesenchymal transition
  • Membrane transfer
  • Metastasis
  • Tumour microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Lis, R., Capdet, J., Mirshahi, P., Lacroix-Triki, M., Dagonnet, F., Klein, C., ... Poupot, M. (2010). Oncologic trogocytosis with Hospicells induces the expression of N-cadherin by breast cancer cells. International Journal of Oncology, 37(6), 1453-1461. https://doi.org/10.3892/ijo-00000797

Oncologic trogocytosis with Hospicells induces the expression of N-cadherin by breast cancer cells. / Lis, Raphael; Capdet, Jérome; Mirshahi, Pejman; Lacroix-Triki, Magali; Dagonnet, Francoise; Klein, Christophe; Mirshahi, Massoud; Fournié, Jean Jacques; Tabrizi, Arash Rafii; Poupot, Mary.

In: International Journal of Oncology, Vol. 37, No. 6, 12.2010, p. 1453-1461.

Research output: Contribution to journalArticle

Lis, R, Capdet, J, Mirshahi, P, Lacroix-Triki, M, Dagonnet, F, Klein, C, Mirshahi, M, Fournié, JJ, Tabrizi, AR & Poupot, M 2010, 'Oncologic trogocytosis with Hospicells induces the expression of N-cadherin by breast cancer cells', International Journal of Oncology, vol. 37, no. 6, pp. 1453-1461. https://doi.org/10.3892/ijo-00000797
Lis, Raphael ; Capdet, Jérome ; Mirshahi, Pejman ; Lacroix-Triki, Magali ; Dagonnet, Francoise ; Klein, Christophe ; Mirshahi, Massoud ; Fournié, Jean Jacques ; Tabrizi, Arash Rafii ; Poupot, Mary. / Oncologic trogocytosis with Hospicells induces the expression of N-cadherin by breast cancer cells. In: International Journal of Oncology. 2010 ; Vol. 37, No. 6. pp. 1453-1461.
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