Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours

Arash Rafii Tabrizi, Pejman Mirshahi, Mary Poupot, Anne Marie Faussat, Anne Simon, Elodie Ducros, Eliane Mery, Bettina Couderc, Raphael Lis, Jerome Capdet, Julie Bergalet, Denis Querleu, Francoise Dagonnet, Jean Jacques Fournié, Jean Pierre Marie, Eric Pujade-Lauraine, Gilles Favre, Jeanine Soria, Massoud Mirshahi

Research output: Contribution to journalArticle

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Abstract

Background: The microenvironment plays a major role in the onset and progression of metastasis. Epithelial ovarian cancer (EOC) tends to metastasize to the peritoneal cavity where interactions within the microenvironment might lead to chemoresistance. Mesothelial cells are important actors of the peritoneal homeostasis; we determined their role in the acquisition of chemoresistance of ovarian tumours. Methodology/Principal Findings: We isolated an original type of stromal cells, referred to as "Hospicells" from ascitis of patients with ovarian carcinosis using limiting dilution. We studied their ability to confer chemoresistance through heterocellular interactions. These stromal cells displayed a new phenotype with positive immunostaining for CD9, CD10, CD29, CD146, CD166 and Multi drug resistance protein. They preferentially interacted with epithelial ovarian cancer cells. This interaction induced chemoresistance to platin and taxans with the implication of multi-drug resistance proteins. This contact enabled EOC cells to capture patches of the Hospicells membrane through oncologic trogocytosis, therefore acquiring their functional P-gp proteins and thus developing chemoresistance. Presence of Hospicells on ovarian cancer tissue micro-array from patients with neo-adjuvant chemotherapy was also significantly associated to chemoresistance. Conclusions/Significance: This is the first report of trogocytosis occurring between a cancer cell and an original type of stromal cell. This interaction induced autonomous acquisition of chemoresistance. The presence of stromal cells within patient's tumour might be predictive of chemoresistance. The specific interaction between cancer cells and stromal cells might be targeted during chemotherapy.

Original languageEnglish
Article numbere3894
JournalPLoS One
Volume3
Issue number12
DOIs
Publication statusPublished - 16 Dec 2008
Externally publishedYes

Fingerprint

ovarian neoplasms
stromal cells
Stromal Cells
Tumors
Chemotherapy
Cells
multiple drug resistance
Multiple Drug Resistance
Neoplasms
Proteins
drug therapy
Pharmaceutical Preparations
Dilution
proteins
Tissue
Membranes
Peritoneal Cavity
Adjuvant Chemotherapy
metastasis
Ovarian Neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tabrizi, A. R., Mirshahi, P., Poupot, M., Faussat, A. M., Simon, A., Ducros, E., ... Mirshahi, M. (2008). Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. PLoS One, 3(12), [e3894]. https://doi.org/10.1371/journal.pone.0003894

Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. / Tabrizi, Arash Rafii; Mirshahi, Pejman; Poupot, Mary; Faussat, Anne Marie; Simon, Anne; Ducros, Elodie; Mery, Eliane; Couderc, Bettina; Lis, Raphael; Capdet, Jerome; Bergalet, Julie; Querleu, Denis; Dagonnet, Francoise; Fournié, Jean Jacques; Marie, Jean Pierre; Pujade-Lauraine, Eric; Favre, Gilles; Soria, Jeanine; Mirshahi, Massoud.

In: PLoS One, Vol. 3, No. 12, e3894, 16.12.2008.

Research output: Contribution to journalArticle

Tabrizi, AR, Mirshahi, P, Poupot, M, Faussat, AM, Simon, A, Ducros, E, Mery, E, Couderc, B, Lis, R, Capdet, J, Bergalet, J, Querleu, D, Dagonnet, F, Fournié, JJ, Marie, JP, Pujade-Lauraine, E, Favre, G, Soria, J & Mirshahi, M 2008, 'Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours', PLoS One, vol. 3, no. 12, e3894. https://doi.org/10.1371/journal.pone.0003894
Tabrizi, Arash Rafii ; Mirshahi, Pejman ; Poupot, Mary ; Faussat, Anne Marie ; Simon, Anne ; Ducros, Elodie ; Mery, Eliane ; Couderc, Bettina ; Lis, Raphael ; Capdet, Jerome ; Bergalet, Julie ; Querleu, Denis ; Dagonnet, Francoise ; Fournié, Jean Jacques ; Marie, Jean Pierre ; Pujade-Lauraine, Eric ; Favre, Gilles ; Soria, Jeanine ; Mirshahi, Massoud. / Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. In: PLoS One. 2008 ; Vol. 3, No. 12.
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AU - Mirshahi, Pejman

AU - Poupot, Mary

AU - Faussat, Anne Marie

AU - Simon, Anne

AU - Ducros, Elodie

AU - Mery, Eliane

AU - Couderc, Bettina

AU - Lis, Raphael

AU - Capdet, Jerome

AU - Bergalet, Julie

AU - Querleu, Denis

AU - Dagonnet, Francoise

AU - Fournié, Jean Jacques

AU - Marie, Jean Pierre

AU - Pujade-Lauraine, Eric

AU - Favre, Gilles

AU - Soria, Jeanine

AU - Mirshahi, Massoud

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N2 - Background: The microenvironment plays a major role in the onset and progression of metastasis. Epithelial ovarian cancer (EOC) tends to metastasize to the peritoneal cavity where interactions within the microenvironment might lead to chemoresistance. Mesothelial cells are important actors of the peritoneal homeostasis; we determined their role in the acquisition of chemoresistance of ovarian tumours. Methodology/Principal Findings: We isolated an original type of stromal cells, referred to as "Hospicells" from ascitis of patients with ovarian carcinosis using limiting dilution. We studied their ability to confer chemoresistance through heterocellular interactions. These stromal cells displayed a new phenotype with positive immunostaining for CD9, CD10, CD29, CD146, CD166 and Multi drug resistance protein. They preferentially interacted with epithelial ovarian cancer cells. This interaction induced chemoresistance to platin and taxans with the implication of multi-drug resistance proteins. This contact enabled EOC cells to capture patches of the Hospicells membrane through oncologic trogocytosis, therefore acquiring their functional P-gp proteins and thus developing chemoresistance. Presence of Hospicells on ovarian cancer tissue micro-array from patients with neo-adjuvant chemotherapy was also significantly associated to chemoresistance. Conclusions/Significance: This is the first report of trogocytosis occurring between a cancer cell and an original type of stromal cell. This interaction induced autonomous acquisition of chemoresistance. The presence of stromal cells within patient's tumour might be predictive of chemoresistance. The specific interaction between cancer cells and stromal cells might be targeted during chemotherapy.

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