Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

Masaru Okuda, Henning Horn, Pheruza Tarapore, Yukari Tokuyama, A. George Smulian, Pui Kwong Chan, Erik S. Knudsen, Irene A. Hofmann, Jean D. Snyder, Kevin E. Bove, Kenji Fukasawa

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Abstract

In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

Original languageEnglish
Pages (from-to)127-140
Number of pages14
JournalCell
Volume103
Issue number1
Publication statusPublished - 29 Sep 2000
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Okuda, M., Horn, H., Tarapore, P., Tokuyama, Y., Smulian, A. G., Chan, P. K., Knudsen, E. S., Hofmann, I. A., Snyder, J. D., Bove, K. E., & Fukasawa, K. (2000). Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication. Cell, 103(1), 127-140.