Nuclear envelope disruption involving host caspases plays a role in the parvovirus replication cycle

Sarah Cohen, Alexandra K. Marr, Pierre Garcin, Nelly Panté

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Parvoviruses are small, nonenveloped, single-stranded DNA viruses which replicate in the nucleus of the host cell. We have previously found that early during infection the parvovirus minute virus of mice (MVM) causes small, transient disruptions of the nuclear envelope (NE). We have now investigated the mechanism used by MVM to disrupt the NE. Here we show that the viral phospholipase A2, the only known enzymatic domain on the parvovirus capsid, is not involved in causing NE disruption. Instead, the virus utilizes host cell caspases, which are proteases involved in causing NE breakdown during apoptosis, to facilitate these nuclear membrane disruptions. Studies with pharmacological inhibitors indicate that caspase-3 in particular is involved. A caspase-3 inhibitor prevents nuclear lamin cleavage and NE disruption in MVM-infected mouse fibroblast cells and reduces nuclear entry of MVM capsids and viral gene expression. Caspase-3 is, however, not activated above basal levels in MVM-infected cells, and other aspects of apoptosis are not triggered during early MVM infection. Instead, basally active caspase-3 is relocalized to the nuclei of infected cells. We propose that NE disruption involving caspases plays a role in (i) parvovirus entry into the nucleus and (ii) alteration of the compartmentalization of host proteins in a way that is favorable for the virus.

Original languageEnglish
Pages (from-to)4863-4874
Number of pages12
JournalJournal of Virology
Volume85
Issue number10
DOIs
Publication statusPublished - May 2011
Externally publishedYes

Fingerprint

Minute Virus of Mice
Parvovirus
Nuclear Envelope
Caspases
Caspase 3
Capsid
Cell Nucleus
Lamins
Apoptosis
Viruses
Parvoviridae Infections
Caspase Inhibitors
DNA Viruses
Viral Genes
Single-Stranded DNA
Phospholipases A2
Virus Diseases
Peptide Hydrolases
Fibroblasts
Pharmacology

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Nuclear envelope disruption involving host caspases plays a role in the parvovirus replication cycle. / Cohen, Sarah; Marr, Alexandra K.; Garcin, Pierre; Panté, Nelly.

In: Journal of Virology, Vol. 85, No. 10, 05.2011, p. 4863-4874.

Research output: Contribution to journalArticle

Cohen, Sarah ; Marr, Alexandra K. ; Garcin, Pierre ; Panté, Nelly. / Nuclear envelope disruption involving host caspases plays a role in the parvovirus replication cycle. In: Journal of Virology. 2011 ; Vol. 85, No. 10. pp. 4863-4874.
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