Novel role of NADPH oxidase in ischemic myocardium: A study with Nox2 knockout mice

Mahesh Thirunavukkarasu, Ram Sudheer Adluri, Bela Juhasz, Samson Mathews Samuel, Lijun Zhan, Anupinder Kaur, Gautam Maulik, Juan A. Sanchez, Janet Hager, Nilanjana Maulik

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Abstract Several potential sources of reactive oxygen species (ROS) in cells exist. One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2 -/- mice. Both wild-type (WT) and Nox2-/- mice were subjected to either 30 min of ischemia followed by 2 h of reperfusion (IR) or IP prior to 30 min ischemia and 2 h of reperfusion. Reduction in left ventricular developed pressure (60.1 versus 63 mmHg), dp/dtmax (893 versus 1,027 mmHg/s), and aortic flow (0.9 versus 1.8 ml/min) was observed in Nox2-/-IPIR compared to WTIPIR along with increased infarct size (33% versus 22%) and apoptosis after 120 min of reperfusion. Differentially regulated genes were demonstrated by comparing gene expression in WTIPIR versus Nox2-/- IPIR hearts. Selected differentially regulated genes such as β-catenin, SRPK3, ERDR1, ACIN1, Syntaxin-8, and STC1 were validated by real-time PCR. Taken together, this is the first report identifying important, differentially expressed genes during ischemic preconditioning in Nox2-/- mice by using microarray analysis.

Original languageEnglish
Pages (from-to)501-514
Number of pages14
JournalFunctional and Integrative Genomics
Volume12
Issue number3
DOIs
Publication statusPublished - 1 Aug 2012
Externally publishedYes

Fingerprint

Ischemic Preconditioning
NADPH Oxidase
Knockout Mice
Reperfusion
Myocardium
Reactive Oxygen Species
Ischemia
Qa-SNARE Proteins
Genes
Catenins
Ventricular Pressure
Microarray Analysis
Superoxides
Real-Time Polymerase Chain Reaction
Apoptosis
Gene Expression

Keywords

  • Cardioprotection
  • Gene expression
  • Ischemic preconditioning
  • NADPH oxidase
  • Nox2

ASJC Scopus subject areas

  • Genetics

Cite this

Novel role of NADPH oxidase in ischemic myocardium : A study with Nox2 knockout mice. / Thirunavukkarasu, Mahesh; Adluri, Ram Sudheer; Juhasz, Bela; Mathews Samuel, Samson; Zhan, Lijun; Kaur, Anupinder; Maulik, Gautam; Sanchez, Juan A.; Hager, Janet; Maulik, Nilanjana.

In: Functional and Integrative Genomics, Vol. 12, No. 3, 01.08.2012, p. 501-514.

Research output: Contribution to journalArticle

Thirunavukkarasu, M, Adluri, RS, Juhasz, B, Mathews Samuel, S, Zhan, L, Kaur, A, Maulik, G, Sanchez, JA, Hager, J & Maulik, N 2012, 'Novel role of NADPH oxidase in ischemic myocardium: A study with Nox2 knockout mice', Functional and Integrative Genomics, vol. 12, no. 3, pp. 501-514. https://doi.org/10.1007/s10142-011-0256-x
Thirunavukkarasu, Mahesh ; Adluri, Ram Sudheer ; Juhasz, Bela ; Mathews Samuel, Samson ; Zhan, Lijun ; Kaur, Anupinder ; Maulik, Gautam ; Sanchez, Juan A. ; Hager, Janet ; Maulik, Nilanjana. / Novel role of NADPH oxidase in ischemic myocardium : A study with Nox2 knockout mice. In: Functional and Integrative Genomics. 2012 ; Vol. 12, No. 3. pp. 501-514.
@article{de9fbcd709524137be2ba94043218cde,
title = "Novel role of NADPH oxidase in ischemic myocardium: A study with Nox2 knockout mice",
abstract = "Abstract Several potential sources of reactive oxygen species (ROS) in cells exist. One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2 -/- mice. Both wild-type (WT) and Nox2-/- mice were subjected to either 30 min of ischemia followed by 2 h of reperfusion (IR) or IP prior to 30 min ischemia and 2 h of reperfusion. Reduction in left ventricular developed pressure (60.1 versus 63 mmHg), dp/dtmax (893 versus 1,027 mmHg/s), and aortic flow (0.9 versus 1.8 ml/min) was observed in Nox2-/-IPIR compared to WTIPIR along with increased infarct size (33{\%} versus 22{\%}) and apoptosis after 120 min of reperfusion. Differentially regulated genes were demonstrated by comparing gene expression in WTIPIR versus Nox2-/- IPIR hearts. Selected differentially regulated genes such as β-catenin, SRPK3, ERDR1, ACIN1, Syntaxin-8, and STC1 were validated by real-time PCR. Taken together, this is the first report identifying important, differentially expressed genes during ischemic preconditioning in Nox2-/- mice by using microarray analysis.",
keywords = "Cardioprotection, Gene expression, Ischemic preconditioning, NADPH oxidase, Nox2",
author = "Mahesh Thirunavukkarasu and Adluri, {Ram Sudheer} and Bela Juhasz and {Mathews Samuel}, Samson and Lijun Zhan and Anupinder Kaur and Gautam Maulik and Sanchez, {Juan A.} and Janet Hager and Nilanjana Maulik",
year = "2012",
month = "8",
day = "1",
doi = "10.1007/s10142-011-0256-x",
language = "English",
volume = "12",
pages = "501--514",
journal = "Functional and Integrative Genomics",
issn = "1438-793X",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Novel role of NADPH oxidase in ischemic myocardium

T2 - A study with Nox2 knockout mice

AU - Thirunavukkarasu, Mahesh

AU - Adluri, Ram Sudheer

AU - Juhasz, Bela

AU - Mathews Samuel, Samson

AU - Zhan, Lijun

AU - Kaur, Anupinder

AU - Maulik, Gautam

AU - Sanchez, Juan A.

AU - Hager, Janet

AU - Maulik, Nilanjana

PY - 2012/8/1

Y1 - 2012/8/1

N2 - Abstract Several potential sources of reactive oxygen species (ROS) in cells exist. One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2 -/- mice. Both wild-type (WT) and Nox2-/- mice were subjected to either 30 min of ischemia followed by 2 h of reperfusion (IR) or IP prior to 30 min ischemia and 2 h of reperfusion. Reduction in left ventricular developed pressure (60.1 versus 63 mmHg), dp/dtmax (893 versus 1,027 mmHg/s), and aortic flow (0.9 versus 1.8 ml/min) was observed in Nox2-/-IPIR compared to WTIPIR along with increased infarct size (33% versus 22%) and apoptosis after 120 min of reperfusion. Differentially regulated genes were demonstrated by comparing gene expression in WTIPIR versus Nox2-/- IPIR hearts. Selected differentially regulated genes such as β-catenin, SRPK3, ERDR1, ACIN1, Syntaxin-8, and STC1 were validated by real-time PCR. Taken together, this is the first report identifying important, differentially expressed genes during ischemic preconditioning in Nox2-/- mice by using microarray analysis.

AB - Abstract Several potential sources of reactive oxygen species (ROS) in cells exist. One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2 -/- mice. Both wild-type (WT) and Nox2-/- mice were subjected to either 30 min of ischemia followed by 2 h of reperfusion (IR) or IP prior to 30 min ischemia and 2 h of reperfusion. Reduction in left ventricular developed pressure (60.1 versus 63 mmHg), dp/dtmax (893 versus 1,027 mmHg/s), and aortic flow (0.9 versus 1.8 ml/min) was observed in Nox2-/-IPIR compared to WTIPIR along with increased infarct size (33% versus 22%) and apoptosis after 120 min of reperfusion. Differentially regulated genes were demonstrated by comparing gene expression in WTIPIR versus Nox2-/- IPIR hearts. Selected differentially regulated genes such as β-catenin, SRPK3, ERDR1, ACIN1, Syntaxin-8, and STC1 were validated by real-time PCR. Taken together, this is the first report identifying important, differentially expressed genes during ischemic preconditioning in Nox2-/- mice by using microarray analysis.

KW - Cardioprotection

KW - Gene expression

KW - Ischemic preconditioning

KW - NADPH oxidase

KW - Nox2

UR - http://www.scopus.com/inward/record.url?scp=84870299120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870299120&partnerID=8YFLogxK

U2 - 10.1007/s10142-011-0256-x

DO - 10.1007/s10142-011-0256-x

M3 - Article

C2 - 22038056

AN - SCOPUS:84870299120

VL - 12

SP - 501

EP - 514

JO - Functional and Integrative Genomics

JF - Functional and Integrative Genomics

SN - 1438-793X

IS - 3

ER -