Novel polymorphic microsatellites for the red-legged partridge (Alectoris rufa) and cross-species amplification in Alectoris graeca

E. G. Gonzalez, A. M. Castilla, R. Zardoya

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The red-legged partridge, Alectoris rufa, is an endemic species of the southwestern Mediterranean, and the most popular game bird in the Iberian Peninsula. A total of 27 microsatellite loci was isolated from an enriched genomic library of A. rufa. Six perfect GT microsatellites were characterized and optimized in 45 individuals of A. rufa. All loci revealed high levels of polymorphism with a number of alleles that ranged from three to 13. Observed heterozygosity ranged from 0.2 to 0.6. Cross-species amplification showed that all loci were also polymorphic in rock partridge, Alectoris graeca. The new markers will be useful in determining hybridization between both species of Alectoris.

Original languageEnglish
Pages (from-to)449-451
Number of pages3
JournalMolecular Ecology Notes
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 2005
Externally publishedYes

Fingerprint

Alectoris
Alectoris rufa
partridges
Microsatellite Repeats
Amplification
amplification
microsatellite repeats
loci
Genomic Library
Birds
Polymorphism
endemic species
heterozygosity
game birds
genomics
allele
polymorphism
Alleles
genomic libraries
Rocks

Keywords

  • Game bird
  • Hybridization
  • Microsatellite
  • Red-legged partridge
  • Rock partridge

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Novel polymorphic microsatellites for the red-legged partridge (Alectoris rufa) and cross-species amplification in Alectoris graeca. / Gonzalez, E. G.; Castilla, A. M.; Zardoya, R.

In: Molecular Ecology Notes, Vol. 5, No. 2, 06.2005, p. 449-451.

Research output: Contribution to journalArticle

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