Novel Pancreas Organogenesis Markers Refine the Pancreatic Differentiation Roadmap of Embryonic Stem cells

Maria Teresa De Angelis, Filomena Russo, Fulvio D'Angelo, Antonella Federico, Marica Gemei, Luigi Del Vecchio, Michele Ceccarelli, Mario De Felice, Geppino Falco

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5 Citations (Scopus)

Abstract

The generation of pancreatic endocrine and exocrine functional precursors from embryonic stem cells (ESCs) is an intriguing opportunity to address cell therapy challenges. The main goal of cellular regeneration is to derive, in vitro, pancreatic progenitor cells (PPCs) that retain the capacity to differentiate following the in vivo developmental ontogeny. In our work, we aim to refine the pancreatic in vitro cellular transitions, through the identification of the intrinsic factors that mark the pancreas budding process at embryonic stage 10.5 (E10.5), in which pancreas precursor specification predominantly occurs. We identified a cohort of genes (Bex1, Nepn, Pcbd1, Prdxdd1, Rnf160, Slc2a1, and Tff3) that marked the pancreas budding genesis, and above all signaled ESC differentiation transitions during pancreatic lineage commitment. Noticeably, we demonstrated that the expression of Nepn marked a naïve pancreatic cellular state that resembled PPC-like specification. Our data considerably improve the comprehension of pancreatic cellular ontogeny, which could be critical for implementing pluripotent stem cells programming and reprogramming toward pancreatic lineage commitment.

Original languageEnglish
Pages (from-to)269-279
Number of pages11
JournalStem Cell Reviews and Reports
Volume10
Issue number2
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

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Keywords

  • Bud
  • Embryonic stem cells
  • Laser microdissection
  • Pancreas
  • Progenitor cells
  • Specification

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology

Cite this

De Angelis, M. T., Russo, F., D'Angelo, F., Federico, A., Gemei, M., Del Vecchio, L., Ceccarelli, M., De Felice, M., & Falco, G. (2014). Novel Pancreas Organogenesis Markers Refine the Pancreatic Differentiation Roadmap of Embryonic Stem cells. Stem Cell Reviews and Reports, 10(2), 269-279. https://doi.org/10.1007/s12015-013-9489-5