Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

E. Elkord, D. J. Burt

Research output: Contribution to journalArticle

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Abstract

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalScandinavian Journal of Immunology
Volume74
Issue number4
DOIs
Publication statusPublished - 1 Oct 2011

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ASJC Scopus subject areas

  • Immunology

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