Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

Eyad Elkord, D. J. Burt

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalScandinavian Journal of Immunology
Volume74
Issue number4
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Fingerprint

Enzyme-Linked Immunospot Assay
Carcinoembryonic Antigen
T-Cell Antigen Receptor
Antigen Receptors
Streptavidin
Neoplasm Antigens
Clinical Trials
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

@article{e8b3bb00052e418194cf34d51336bc58,
title = "Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells",
abstract = "We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.",
author = "Eyad Elkord and Burt, {D. J.}",
year = "2011",
month = "10",
doi = "10.1111/j.1365-3083.2011.02588.x",
language = "English",
volume = "74",
pages = "419--422",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

AU - Elkord, Eyad

AU - Burt, D. J.

PY - 2011/10

Y1 - 2011/10

N2 - We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

AB - We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

UR - http://www.scopus.com/inward/record.url?scp=80052691211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052691211&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3083.2011.02588.x

DO - 10.1111/j.1365-3083.2011.02588.x

M3 - Article

VL - 74

SP - 419

EP - 422

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 4

ER -