Abstract
We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
| Original language | English |
|---|---|
| Pages (from-to) | 419-422 |
| Number of pages | 4 |
| Journal | Scandinavian Journal of Immunology |
| Volume | 74 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 2011 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells. / Elkord, Eyad; Burt, D. J.
In: Scandinavian Journal of Immunology, Vol. 74, No. 4, 10.2011, p. 419-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells
AU - Elkord, Eyad
AU - Burt, D. J.
PY - 2011/10
Y1 - 2011/10
N2 - We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
AB - We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
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UR - http://www.scopus.com/inward/citedby.url?scp=80052691211&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3083.2011.02588.x
DO - 10.1111/j.1365-3083.2011.02588.x
M3 - Article
C2 - 21645031
AN - SCOPUS:80052691211
VL - 74
SP - 419
EP - 422
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
SN - 0300-9475
IS - 4
ER -