Noninfectious X4 but not R5 human immunodeficiency virus type 1 virions inhibit humoral immune responses in human lymphoid tissue ex vivo

Wendy Fitzgerald, Andrew W. Sylwester, Jean-Charles B. Grivel, Jeffrey D. Lifson, Leonid B. Margolis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ex vivo human immunodeficiency virus type 1 (HIV-1) infection of human lymphoid tissue recapitulates some aspects of in vivo HIV-1 infection, including a severe depletion of CD4+ T cells and suppression of humoral immune responses to recall antigens or to polyclonal stimuli. These effects are induced by infection with X4 HIV-1 variants, whereas infection with R5 variants results in only mild depletion of CD4+ T cells and no suppression of immune responses. To study the mechanisms of suppression of immune responses in this ex vivo system, we used aldrithiol-2 (AT-2)-inactivated virions that have functional envelope glycoproteins but are not infectious and do not deplete CD4+ T cells in human lymphoid tissues ex vivo. Nevertheless, AT-2-inactivated X4 (but not R5) HIV-1 virions, even with only a brief exposure, inhibit antibody responses in human lymphoid tissue ex vivo, similarly to infectious virus. This phenomenon is mediated by soluble immunosuppressive factor(s) secreted by tissue exposed to virus.

Original languageEnglish
Pages (from-to)7061-7068
Number of pages8
JournalJournal of Virology
Volume78
Issue number13
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

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Lymphoid Tissue
Humoral Immunity
Virion
HIV-1
Virus Diseases
T-Lymphocytes
Viruses
Immunosuppressive Agents
Infection
Antibody Formation
Glycoproteins
Antigens
2,2'-dipyridyl disulfide

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Noninfectious X4 but not R5 human immunodeficiency virus type 1 virions inhibit humoral immune responses in human lymphoid tissue ex vivo. / Fitzgerald, Wendy; Sylwester, Andrew W.; Grivel, Jean-Charles B.; Lifson, Jeffrey D.; Margolis, Leonid B.

In: Journal of Virology, Vol. 78, No. 13, 07.2004, p. 7061-7068.

Research output: Contribution to journalArticle

Fitzgerald, Wendy ; Sylwester, Andrew W. ; Grivel, Jean-Charles B. ; Lifson, Jeffrey D. ; Margolis, Leonid B. / Noninfectious X4 but not R5 human immunodeficiency virus type 1 virions inhibit humoral immune responses in human lymphoid tissue ex vivo. In: Journal of Virology. 2004 ; Vol. 78, No. 13. pp. 7061-7068.
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