Next generation sequencing

New tools in immunology and hematology

Antonio Mori, Sara Deola, Luciano Xumerle, Vladan Mijatovic, Giovanni Malerba, Vladia Monsurrò

Research output: Contribution to journalReview article

27 Citations (Scopus)

Abstract

One of the hallmarks of the adaptive immune system is the specificity of B and T cell receptors. Thanks to somatic recombination, a large repertoire of receptors can be generated within an individual that guarantee the recognition of a vast number of antigens. Monoclonal antibodies have limited applicability, given the high degree of diversity among these receptors, in BCR and TCR monitoring. Furthermore, with regard to cancer, better characterization of complex genomes and the ability to monitor tumor-specific cryptic mutations or translocations are needed to develop better tailored therapies. Novel technologies, by enhancing the ability of BCR and TCR monitoring, can help in the search for minimal residual disease during hematological malignancy diagnosis and follow-up, and can aid in improving bone marrow transplantation techniques. Recently, a novel technology known as next generation sequencing has been developed; this allows the recognition of unique sequences and provides depth of coverage, heterogeneity, and accuracy of sequencing. This provides a powerful tool that, along with microarray analysis for gene expression, may become integral in resolving the remaining key problems in hematology. This review describes the state of the art of this novel technology, its application in the immunological and hematological fields, and the possible benefits it will provide for the hematology and immunology community.

Original languageEnglish
Pages (from-to)242-249
Number of pages8
JournalBlood Research
Volume48
Issue number4
DOIs
Publication statusPublished - 1 Dec 2013
Externally publishedYes

Fingerprint

Hematology
Allergy and Immunology
Technology
Residual Neoplasm
Hematologic Neoplasms
Microarray Analysis
T-Cell Antigen Receptor
Bone Marrow Transplantation
Genetic Recombination
Immune System
Neoplasms
B-Lymphocytes
Monoclonal Antibodies
Genome
Gene Expression
Antigens
Mutation
Therapeutics

Keywords

  • B cell receptor
  • Immune monitoring
  • Next generation sequence
  • T cell receptor

ASJC Scopus subject areas

  • Hematology

Cite this

Mori, A., Deola, S., Xumerle, L., Mijatovic, V., Malerba, G., & Monsurrò, V. (2013). Next generation sequencing: New tools in immunology and hematology. Blood Research, 48(4), 242-249. https://doi.org/10.5045/br.2013.48.4.242

Next generation sequencing : New tools in immunology and hematology. / Mori, Antonio; Deola, Sara; Xumerle, Luciano; Mijatovic, Vladan; Malerba, Giovanni; Monsurrò, Vladia.

In: Blood Research, Vol. 48, No. 4, 01.12.2013, p. 242-249.

Research output: Contribution to journalReview article

Mori, A, Deola, S, Xumerle, L, Mijatovic, V, Malerba, G & Monsurrò, V 2013, 'Next generation sequencing: New tools in immunology and hematology', Blood Research, vol. 48, no. 4, pp. 242-249. https://doi.org/10.5045/br.2013.48.4.242
Mori A, Deola S, Xumerle L, Mijatovic V, Malerba G, Monsurrò V. Next generation sequencing: New tools in immunology and hematology. Blood Research. 2013 Dec 1;48(4):242-249. https://doi.org/10.5045/br.2013.48.4.242
Mori, Antonio ; Deola, Sara ; Xumerle, Luciano ; Mijatovic, Vladan ; Malerba, Giovanni ; Monsurrò, Vladia. / Next generation sequencing : New tools in immunology and hematology. In: Blood Research. 2013 ; Vol. 48, No. 4. pp. 242-249.
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