Neutrophil P-selectin-glycoprotein-ligand-I binding to platelet P-selectin enhances metalloproteinase 2 secretion and platelet-neutrophil aggregation

Haissam Abou-Saleh, Jean François Théorêt, Daniel Yacoub, Yahye Merhi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Platelets and neutrophils constitute a high source of metalloproteinases (MMPs), and their interactions via P-selectin and P-selectin-glycoprotein-ligand-1 (PSGL-1) are involved in thrombosis, vascular remodelling, and restenosis. We investigated the impact of these interactions on platelet MMP-2 secretion and function in platelet and neutrophil aggregation. The secretion of MMP-2 from human platelets was significantly increased three-fold after thrombin activation, and enhanced two-fold in the presence of neutrophils. Neutrophil supernatant had no effect on platelet MMP-2 secretion. While no MMP-2 was detected in the supernatant of neutrophils, a high amount of MMP-9 was released by neutrophils, and remained unchanged upon thrombin activation or in the presence of platelets. Platelet P-selectin, which increased significantly after activation, triggered platelet binding to neutrophils that was completely inhibited by P-selectin or PSGL-1 antagonists, and was reduced by 50% with a GPIIb/IIIa antagonist. P-selectin or PSGL-1 antagonism abolished the enhanced secretion of platelet MMP-2 in the presence of neutrophils and reduced platelet-neutrophil aggregation. Platelet activation and binding to neutrophils enhance the secretion of platelet MMP-2 via an adhesive interaction between P-selectin and PSGL-1, which contribute to increase platelet-neutrophil aggregation.

Original languageEnglish
Pages (from-to)1230-1235
Number of pages6
JournalThrombosis and Haemostasis
Volume94
Issue number6
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

Fingerprint

P-Selectin
P-Glycoprotein
Metalloproteases
Platelet Aggregation
Neutrophils
Blood Platelets
Matrix Metalloproteinases
Ligands
Platelet Activation
Thrombin
Adhesives
Thrombosis

Keywords

  • Cell adhesion molecules
  • Leukocytes
  • Metalloproteinases
  • Platelets

ASJC Scopus subject areas

  • Hematology

Cite this

Neutrophil P-selectin-glycoprotein-ligand-I binding to platelet P-selectin enhances metalloproteinase 2 secretion and platelet-neutrophil aggregation. / Abou-Saleh, Haissam; Théorêt, Jean François; Yacoub, Daniel; Merhi, Yahye.

In: Thrombosis and Haemostasis, Vol. 94, No. 6, 12.2005, p. 1230-1235.

Research output: Contribution to journalArticle

Abou-Saleh, Haissam ; Théorêt, Jean François ; Yacoub, Daniel ; Merhi, Yahye. / Neutrophil P-selectin-glycoprotein-ligand-I binding to platelet P-selectin enhances metalloproteinase 2 secretion and platelet-neutrophil aggregation. In: Thrombosis and Haemostasis. 2005 ; Vol. 94, No. 6. pp. 1230-1235.
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