Neurobehavioral development of two mouse lines commonly used in transgenic studies

M. Dierssen, V. Fotaki, M. Martínez de Lagrán, M. Gratacós, M. Arbonés, C. Fillat, Xavier P. Estivill

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The present study was aimed at establishing the differences in the neurodevelopmental profile between two F2 lines derived from two F1 hybrid mouse strains (129×C57BL/6 and C57BL/6×SJL). The choice of the given strains was based on the frequent use of these mice in transgenic research. For the neurodevelopment phenotyping, we employed a test battery consisting of 23 somatometric, sensorial and motor tests. Significant variations between the strains were established in different functional domains. Some specific delays in the appearance of developmental landmarks were observed in F2 mice derived from crosses of F1 C57BL/6×129, whereas they acquired early developmental functions, such as the righting reflex, sooner than C57BL/6×SJL-derived mice. C57BL/6×129 F2 offspring were spontaneously hypoactive, and their poorer motor performance was confirmed by low performance in the negative geotaxis test. However, there were no differences in the general psychomotor development as shown by the good performance in the homing test in both F2 lines. Both strains were susceptible to the handling procedures used, presenting a similar alteration in the response observed in the homing test as compared to nonhandled control mice. In conclusion, our work highlights the importance of the genetic background for transgenesis experiments and also the need for well-established testing protocols to obtain sufficient information at the first stage of behavioral screening of genetically modified mice.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalPharmacology Biochemistry and Behavior
Volume73
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Righting Reflex
Gene Transfer Techniques
Screening
Transgenic Mice
Testing
Research
Experiments
Genetic Background

Keywords

  • Genetic background
  • Genetically modified mice
  • Neurodevelopment

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

Cite this

Neurobehavioral development of two mouse lines commonly used in transgenic studies. / Dierssen, M.; Fotaki, V.; Martínez de Lagrán, M.; Gratacós, M.; Arbonés, M.; Fillat, C.; Estivill, Xavier P.

In: Pharmacology Biochemistry and Behavior, Vol. 73, No. 1, 2002, p. 19-25.

Research output: Contribution to journalArticle

Dierssen, M, Fotaki, V, Martínez de Lagrán, M, Gratacós, M, Arbonés, M, Fillat, C & Estivill, XP 2002, 'Neurobehavioral development of two mouse lines commonly used in transgenic studies', Pharmacology Biochemistry and Behavior, vol. 73, no. 1, pp. 19-25. https://doi.org/10.1016/S0091-3057(02)00792-X
Dierssen, M. ; Fotaki, V. ; Martínez de Lagrán, M. ; Gratacós, M. ; Arbonés, M. ; Fillat, C. ; Estivill, Xavier P. / Neurobehavioral development of two mouse lines commonly used in transgenic studies. In: Pharmacology Biochemistry and Behavior. 2002 ; Vol. 73, No. 1. pp. 19-25.
@article{b6907ed851064f07bf60a4e415106e1c,
title = "Neurobehavioral development of two mouse lines commonly used in transgenic studies",
abstract = "The present study was aimed at establishing the differences in the neurodevelopmental profile between two F2 lines derived from two F1 hybrid mouse strains (129×C57BL/6 and C57BL/6×SJL). The choice of the given strains was based on the frequent use of these mice in transgenic research. For the neurodevelopment phenotyping, we employed a test battery consisting of 23 somatometric, sensorial and motor tests. Significant variations between the strains were established in different functional domains. Some specific delays in the appearance of developmental landmarks were observed in F2 mice derived from crosses of F1 C57BL/6×129, whereas they acquired early developmental functions, such as the righting reflex, sooner than C57BL/6×SJL-derived mice. C57BL/6×129 F2 offspring were spontaneously hypoactive, and their poorer motor performance was confirmed by low performance in the negative geotaxis test. However, there were no differences in the general psychomotor development as shown by the good performance in the homing test in both F2 lines. Both strains were susceptible to the handling procedures used, presenting a similar alteration in the response observed in the homing test as compared to nonhandled control mice. In conclusion, our work highlights the importance of the genetic background for transgenesis experiments and also the need for well-established testing protocols to obtain sufficient information at the first stage of behavioral screening of genetically modified mice.",
keywords = "Genetic background, Genetically modified mice, Neurodevelopment",
author = "M. Dierssen and V. Fotaki and {Mart{\'i}nez de Lagr{\'a}n}, M. and M. Gratac{\'o}s and M. Arbon{\'e}s and C. Fillat and Estivill, {Xavier P.}",
year = "2002",
doi = "10.1016/S0091-3057(02)00792-X",
language = "English",
volume = "73",
pages = "19--25",
journal = "Pharmacology Biochemistry and Behavior",
issn = "0091-3057",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Neurobehavioral development of two mouse lines commonly used in transgenic studies

AU - Dierssen, M.

AU - Fotaki, V.

AU - Martínez de Lagrán, M.

AU - Gratacós, M.

AU - Arbonés, M.

AU - Fillat, C.

AU - Estivill, Xavier P.

PY - 2002

Y1 - 2002

N2 - The present study was aimed at establishing the differences in the neurodevelopmental profile between two F2 lines derived from two F1 hybrid mouse strains (129×C57BL/6 and C57BL/6×SJL). The choice of the given strains was based on the frequent use of these mice in transgenic research. For the neurodevelopment phenotyping, we employed a test battery consisting of 23 somatometric, sensorial and motor tests. Significant variations between the strains were established in different functional domains. Some specific delays in the appearance of developmental landmarks were observed in F2 mice derived from crosses of F1 C57BL/6×129, whereas they acquired early developmental functions, such as the righting reflex, sooner than C57BL/6×SJL-derived mice. C57BL/6×129 F2 offspring were spontaneously hypoactive, and their poorer motor performance was confirmed by low performance in the negative geotaxis test. However, there were no differences in the general psychomotor development as shown by the good performance in the homing test in both F2 lines. Both strains were susceptible to the handling procedures used, presenting a similar alteration in the response observed in the homing test as compared to nonhandled control mice. In conclusion, our work highlights the importance of the genetic background for transgenesis experiments and also the need for well-established testing protocols to obtain sufficient information at the first stage of behavioral screening of genetically modified mice.

AB - The present study was aimed at establishing the differences in the neurodevelopmental profile between two F2 lines derived from two F1 hybrid mouse strains (129×C57BL/6 and C57BL/6×SJL). The choice of the given strains was based on the frequent use of these mice in transgenic research. For the neurodevelopment phenotyping, we employed a test battery consisting of 23 somatometric, sensorial and motor tests. Significant variations between the strains were established in different functional domains. Some specific delays in the appearance of developmental landmarks were observed in F2 mice derived from crosses of F1 C57BL/6×129, whereas they acquired early developmental functions, such as the righting reflex, sooner than C57BL/6×SJL-derived mice. C57BL/6×129 F2 offspring were spontaneously hypoactive, and their poorer motor performance was confirmed by low performance in the negative geotaxis test. However, there were no differences in the general psychomotor development as shown by the good performance in the homing test in both F2 lines. Both strains were susceptible to the handling procedures used, presenting a similar alteration in the response observed in the homing test as compared to nonhandled control mice. In conclusion, our work highlights the importance of the genetic background for transgenesis experiments and also the need for well-established testing protocols to obtain sufficient information at the first stage of behavioral screening of genetically modified mice.

KW - Genetic background

KW - Genetically modified mice

KW - Neurodevelopment

UR - http://www.scopus.com/inward/record.url?scp=0035985524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035985524&partnerID=8YFLogxK

U2 - 10.1016/S0091-3057(02)00792-X

DO - 10.1016/S0091-3057(02)00792-X

M3 - Article

VL - 73

SP - 19

EP - 25

JO - Pharmacology Biochemistry and Behavior

JF - Pharmacology Biochemistry and Behavior

SN - 0091-3057

IS - 1

ER -