Nanomagnetic immunoassay for rapid detection of Escherichia coli

Y. Haik, A. Qablan, C. J. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study identifies a simple process and a hand held device to quickly conduct an analysis, under field conditions, for detection of pathogens. Hand held devices for rapid detection of pathogens have been proposed to be used by first responders. A magnetic sandwich immunoassay is developed for rapid isolation and detection of targeted pathogen. Escherichia coli BL21 Star (DE3) pLysS is used as a model in this study. The magnetic immunoassay takes advantage of the multiple epitopes that are found on the target. On one epitope a magnetic particle is attached. On another epitope a glucose molecule is attached. Glucometers have been designed with suitable accuracy to detect small amounts of glucose through the oxidation of glucose to gluconic acid at the site of platinum electrode. The sandwich magnetic assay that contains the glucose label can be easily correlated to the concentration of the Escherichia coli BL21 Star (DE3) pLysS.

Original languageEnglish
Title of host publication2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings
EditorsM. Laudon, B. Romanowicz
Pages81-84
Number of pages4
Publication statusPublished - 1 Dec 2005
Event2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 - Anaheim, CA, United States
Duration: 8 May 200512 May 2005

Publication series

Name2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings

Other

Other2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005
CountryUnited States
CityAnaheim, CA
Period8/5/0512/5/05

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Keywords

  • E.coli
  • Glucose
  • Magnetic immunoassay
  • Nanoparticles

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Haik, Y., Qablan, A., & Chen, C. J. (2005). Nanomagnetic immunoassay for rapid detection of Escherichia coli. In M. Laudon, & B. Romanowicz (Eds.), 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings (pp. 81-84). (2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings).