NAADP mobilizes calcium from acidic organelles through two-pore channels

Peter J. Calcraft, Margarida Ruas, Zui Pan, Xiaotong Cheng, Abdelilah Arredouani, Xuemei Hao, Jisen Tang, Katja Rietdorf, Lydia Teboul, Kai Ting Chuang, Peihui Lin, Rui Xiao, Chunbo Wang, Yingmin Zhu, Yakang Lin, Christopher N. Wyatt, John Parrington, Jianjie Ma, A. Mark Evans, Antony GalioneMichael X. Zhu

Research output: Contribution to journalArticle

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Abstract

Ca 2+ mobilization from intracellular stores represents an important cell signalling process that is regulated, in mammalian cells, by inositol-1,4,5-trisphosphate (InsP 3), cyclic ADP ribose and nicotinic acid adenine dinucleotide phosphate (NAADP). InsP 3 and cyclic ADP ribose cause the release of Ca 2+ from sarcoplasmic/endoplasmic reticulum stores by the activation of InsP 3 and ryanodine receptors (InsP 3 Rs and RyRs). In contrast, the nature of the intracellular stores targeted by NAADP and the molecular identity of the NAADP receptors remain controversial, although evidence indicates that NAADP mobilizes Ca 2+ from lysosome-related acidic compartments. Here we show that two-pore channels (TPCs) comprise a family of NAADP receptors, with human TPC1 (also known as TPCN1) and chicken TPC3 (TPCN3) being expressed on endosomal membranes, and human TPC2 (TPCN2) on lysosomal membranes when expressed in HEK293 cells. Membranes enriched with TPC2 show high affinity NAADP binding, and TPC2 underpins NAADP-induced Ca 2+ release from lysosome-related stores that is subsequently amplified by Ca 2+-induced Ca 2+ release by InsP 3 Rs. Responses to NAADP were abolished by disrupting the lysosomal proton gradient and by ablating TPC2 expression, but were only attenuated by depleting endoplasmic reticulum Ca 2+ stores or by blocking InsP 3 Rs. Thus, TPCs form NAADP receptors that release Ca 2+ from acidic organelles, which can trigger further Ca 2+ signals via sarcoplasmic/endoplasmic reticulum. TPCs therefore provide new insights into the regulation and organization of Ca 2+ signals in animal cells, and will advance our understanding of the physiological role of NAADP.

Original languageEnglish
Pages (from-to)596-600
Number of pages5
JournalNature
Volume459
Issue number7246
DOIs
Publication statusPublished - 28 May 2009
Externally publishedYes

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Organelles
Calcium
Cyclic ADP-Ribose
Endoplasmic Reticulum
Sarcoplasmic Reticulum
Lysosomes
Membranes
NAADP
Ryanodine Receptor Calcium Release Channel
Inositol 1,4,5-Trisphosphate
HEK293 Cells
Protons
Chickens

ASJC Scopus subject areas

  • General

Cite this

Calcraft, P. J., Ruas, M., Pan, Z., Cheng, X., Arredouani, A., Hao, X., ... Zhu, M. X. (2009). NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature, 459(7246), 596-600. https://doi.org/10.1038/nature08030

NAADP mobilizes calcium from acidic organelles through two-pore channels. / Calcraft, Peter J.; Ruas, Margarida; Pan, Zui; Cheng, Xiaotong; Arredouani, Abdelilah; Hao, Xuemei; Tang, Jisen; Rietdorf, Katja; Teboul, Lydia; Chuang, Kai Ting; Lin, Peihui; Xiao, Rui; Wang, Chunbo; Zhu, Yingmin; Lin, Yakang; Wyatt, Christopher N.; Parrington, John; Ma, Jianjie; Evans, A. Mark; Galione, Antony; Zhu, Michael X.

In: Nature, Vol. 459, No. 7246, 28.05.2009, p. 596-600.

Research output: Contribution to journalArticle

Calcraft, PJ, Ruas, M, Pan, Z, Cheng, X, Arredouani, A, Hao, X, Tang, J, Rietdorf, K, Teboul, L, Chuang, KT, Lin, P, Xiao, R, Wang, C, Zhu, Y, Lin, Y, Wyatt, CN, Parrington, J, Ma, J, Evans, AM, Galione, A & Zhu, MX 2009, 'NAADP mobilizes calcium from acidic organelles through two-pore channels', Nature, vol. 459, no. 7246, pp. 596-600. https://doi.org/10.1038/nature08030
Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X et al. NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature. 2009 May 28;459(7246):596-600. https://doi.org/10.1038/nature08030
Calcraft, Peter J. ; Ruas, Margarida ; Pan, Zui ; Cheng, Xiaotong ; Arredouani, Abdelilah ; Hao, Xuemei ; Tang, Jisen ; Rietdorf, Katja ; Teboul, Lydia ; Chuang, Kai Ting ; Lin, Peihui ; Xiao, Rui ; Wang, Chunbo ; Zhu, Yingmin ; Lin, Yakang ; Wyatt, Christopher N. ; Parrington, John ; Ma, Jianjie ; Evans, A. Mark ; Galione, Antony ; Zhu, Michael X. / NAADP mobilizes calcium from acidic organelles through two-pore channels. In: Nature. 2009 ; Vol. 459, No. 7246. pp. 596-600.
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AU - Hao, Xuemei

AU - Tang, Jisen

AU - Rietdorf, Katja

AU - Teboul, Lydia

AU - Chuang, Kai Ting

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AU - Wang, Chunbo

AU - Zhu, Yingmin

AU - Lin, Yakang

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