Myocardial Phosphoinositides Do Not Share the Same Fatty Acid Profile

J. M.J. Lamers, D. H.W. Dekkers, Nasrin Mesaeli, V. Panagia, H. A.A. Vanheugten

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

It is generally assumed that the fatty acid compositions of the phosphoinositides are identical. To investigate this in myocardium, inositol lipids extracted from rat and pig ventricular homogenates were absorbed to neomycin-coated glass heads, eluted and quantitated by fatty acid analysis after thin-layer chromatography. The percentages of stearic, oleic, linoleic and arachidonic acid (20:4n-6) in the rat were, respectively, 49, 4, 7 and 26 for phosphatidylinositol, 62, 1, 4 and 18 for phosphatidylinositol-4-monophosphate and 63,2,4, 18 for phosphatidylinositol-4,5-bisphosphate. Equal distribution patterns of fatty acids were found in homogenate and sarcoplasmic reticulum of pig myocardium. Cultured rat ventricular myocytes were utilized to study the incorporation (25 h) of [14C]20:4n-6 relative to that of myo-[3H]inositol into phosphatidylinositol and phosphatidylinositol-4,5-bisphosphate which were, respectively, 1.61 and 1.22. The data indicate that in myocardium phosphatidylinositol-4,5-bisphosphate represents a relatively modest source of 20:4n-6.

Original languageEnglish
Pages (from-to)487-494
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number2
DOIs
Publication statusPublished - 15 Mar 1993
Externally publishedYes

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Phosphatidylinositols
Fatty Acids
Rats
Myocardium
Inositol
Swine
Thin layer chromatography
Neomycin
Sarcoplasmic Reticulum
Thin Layer Chromatography
Muscle Cells
Glass
Head
Lipids
Chemical analysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Myocardial Phosphoinositides Do Not Share the Same Fatty Acid Profile. / Lamers, J. M.J.; Dekkers, D. H.W.; Mesaeli, Nasrin; Panagia, V.; Vanheugten, H. A.A.

In: Biochemical and Biophysical Research Communications, Vol. 191, No. 2, 15.03.1993, p. 487-494.

Research output: Contribution to journalArticle

Lamers, J. M.J. ; Dekkers, D. H.W. ; Mesaeli, Nasrin ; Panagia, V. ; Vanheugten, H. A.A. / Myocardial Phosphoinositides Do Not Share the Same Fatty Acid Profile. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 191, No. 2. pp. 487-494.
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