Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families

T. Casals, V. Nunes, C. Lázaro, F. J. Giménez, E. Girbau, V. Volpini, Xavier P. Estivill

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have analysed haplotypes for four DNA polymorphisms, closely linked to the cystic fibrosis (CF) gene, in 82 Spanish families, in which the CF probands are either homozygous for non-AF508 mutations or heterozygous for the AF508 deletion and other CF mutations. The analysis provides genetic data for a new polymorphism for the closely linked marker p KM. 19, which is very strongly associated with CF. Haplotypes generated with the four marker loci are also in strong disequilibrium with the non-AF508 CF chromosomes. The data reported here are useful in 1 in 4 risk pregnancies of parents who have no living affected child, and when counselling close relatives of CF families who are negative for the major CF mutation. The data presented are useful in our population, in which the majority of CF mutations, apart from the AFSOS deletion, are uncommon. For other populations in which mutation heterogeneity is also very high, it still might be more feasible to use RFLPs for diagnostic purposes, when analysis for common mutations is negative and DNA is available from the index patient. The experience presented here provides a model for these population groups who in turn should obtain their own haplotype data. In addition, the model system for genetic counselling presented here might also be useful for other genetic disorders.

Original languageEnglish
Pages (from-to)771-776
Number of pages6
JournalJournal of Medical Genetics
Volume28
Issue number11
Publication statusPublished - Nov 1991
Externally publishedYes

Fingerprint

Linkage Disequilibrium
Genetic Counseling
Cystic Fibrosis
counseling
Mutation
Haplotypes
population group
system model
pregnancy
diagnostic
parents
Inborn Genetic Diseases
DNA
Population Groups
Restriction Fragment Length Polymorphisms
Population
Counseling
experience
Chromosomes
Parents

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Public Health, Environmental and Occupational Health
  • Health(social science)

Cite this

Casals, T., Nunes, V., Lázaro, C., Giménez, F. J., Girbau, E., Volpini, V., & Estivill, X. P. (1991). Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families. Journal of Medical Genetics, 28(11), 771-776.

Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families. / Casals, T.; Nunes, V.; Lázaro, C.; Giménez, F. J.; Girbau, E.; Volpini, V.; Estivill, Xavier P.

In: Journal of Medical Genetics, Vol. 28, No. 11, 11.1991, p. 771-776.

Research output: Contribution to journalArticle

Casals, T, Nunes, V, Lázaro, C, Giménez, FJ, Girbau, E, Volpini, V & Estivill, XP 1991, 'Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families', Journal of Medical Genetics, vol. 28, no. 11, pp. 771-776.
Casals T, Nunes V, Lázaro C, Giménez FJ, Girbau E, Volpini V et al. Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families. Journal of Medical Genetics. 1991 Nov;28(11):771-776.
Casals, T. ; Nunes, V. ; Lázaro, C. ; Giménez, F. J. ; Girbau, E. ; Volpini, V. ; Estivill, Xavier P. / Mutation and linkage disequilibrium analysis in genetic counselling of Spanish cystic fibrosis families. In: Journal of Medical Genetics. 1991 ; Vol. 28, No. 11. pp. 771-776.
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