Multiplex polymerase chain reaction for detection of gastrointestinal pathogens in migrant workers in Qatar

John M. Humphrey, Sanjay Ranbhise, Emad Ibrahim, Hamad E. Al-Romaihi, Elmoubasher Farag, Laith Aburaddad, Marshall J. Glesby

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015-2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray Gastrointestinal PCR). A target pathogen was identified in 62.7% of subjects. Enteropathogenic Escherichia coli was the most prevalent pathogen and was detected in 24.6% of subjects, followed by Salmonella (22.2%), enteroaggregative E. coli (15.1%), Giardia lamblia (9.5%), and enterotoxigenic E. coli (8.7%). Multiple pathogens were identified in 49.3% of positive stool samples. In a multivariable analysis, the presence of a heart rate ? 90 (adjusted odds ratio [OR] = 3.7, 95% confidence interval [CI] = 1.4-10.0) and > 5 fecal leukocytes/high-power field (adjusted OR = 2.8, 95% CI = 1.2-7.0) were significant predictors of detecting an acute inflammatory pathogen by PCR. Use of multiplex PCR enabled the detection of gastrointestinal pathogens in a high proportion of cases, illustrating the utility of this diagnostic tool in epidemiologic studies of infectious diarrhea.

Original languageEnglish
Pages (from-to)1330-1337
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume95
Issue number6
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

Fingerprint

Qatar
Multiplex Polymerase Chain Reaction
Diarrhea
Odds Ratio
Confidence Intervals
Red Cross
Enterotoxigenic Escherichia coli
Enteropathogenic Escherichia coli
Giardia lamblia
Polymerase Chain Reaction
Ambulatory Care Facilities
Salmonella
Observational Studies
Hospital Emergency Service
Epidemiologic Studies
Leukocytes
Heart Rate
Demography
Prospective Studies
Escherichia coli

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Multiplex polymerase chain reaction for detection of gastrointestinal pathogens in migrant workers in Qatar. / Humphrey, John M.; Ranbhise, Sanjay; Ibrahim, Emad; Al-Romaihi, Hamad E.; Farag, Elmoubasher; Aburaddad, Laith; Glesby, Marshall J.

In: American Journal of Tropical Medicine and Hygiene, Vol. 95, No. 6, 01.12.2016, p. 1330-1337.

Research output: Contribution to journalArticle

Humphrey, John M. ; Ranbhise, Sanjay ; Ibrahim, Emad ; Al-Romaihi, Hamad E. ; Farag, Elmoubasher ; Aburaddad, Laith ; Glesby, Marshall J. / Multiplex polymerase chain reaction for detection of gastrointestinal pathogens in migrant workers in Qatar. In: American Journal of Tropical Medicine and Hygiene. 2016 ; Vol. 95, No. 6. pp. 1330-1337.
@article{85936c60a40f480b8ebe5a820d2a5079,
title = "Multiplex polymerase chain reaction for detection of gastrointestinal pathogens in migrant workers in Qatar",
abstract = "The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015-2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray Gastrointestinal PCR). A target pathogen was identified in 62.7{\%} of subjects. Enteropathogenic Escherichia coli was the most prevalent pathogen and was detected in 24.6{\%} of subjects, followed by Salmonella (22.2{\%}), enteroaggregative E. coli (15.1{\%}), Giardia lamblia (9.5{\%}), and enterotoxigenic E. coli (8.7{\%}). Multiple pathogens were identified in 49.3{\%} of positive stool samples. In a multivariable analysis, the presence of a heart rate ? 90 (adjusted odds ratio [OR] = 3.7, 95{\%} confidence interval [CI] = 1.4-10.0) and > 5 fecal leukocytes/high-power field (adjusted OR = 2.8, 95{\%} CI = 1.2-7.0) were significant predictors of detecting an acute inflammatory pathogen by PCR. Use of multiplex PCR enabled the detection of gastrointestinal pathogens in a high proportion of cases, illustrating the utility of this diagnostic tool in epidemiologic studies of infectious diarrhea.",
author = "Humphrey, {John M.} and Sanjay Ranbhise and Emad Ibrahim and Al-Romaihi, {Hamad E.} and Elmoubasher Farag and Laith Aburaddad and Glesby, {Marshall J.}",
year = "2016",
month = "12",
day = "1",
doi = "10.4269/ajtmh.16-0464",
language = "English",
volume = "95",
pages = "1330--1337",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "6",

}

TY - JOUR

T1 - Multiplex polymerase chain reaction for detection of gastrointestinal pathogens in migrant workers in Qatar

AU - Humphrey, John M.

AU - Ranbhise, Sanjay

AU - Ibrahim, Emad

AU - Al-Romaihi, Hamad E.

AU - Farag, Elmoubasher

AU - Aburaddad, Laith

AU - Glesby, Marshall J.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015-2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray Gastrointestinal PCR). A target pathogen was identified in 62.7% of subjects. Enteropathogenic Escherichia coli was the most prevalent pathogen and was detected in 24.6% of subjects, followed by Salmonella (22.2%), enteroaggregative E. coli (15.1%), Giardia lamblia (9.5%), and enterotoxigenic E. coli (8.7%). Multiple pathogens were identified in 49.3% of positive stool samples. In a multivariable analysis, the presence of a heart rate ? 90 (adjusted odds ratio [OR] = 3.7, 95% confidence interval [CI] = 1.4-10.0) and > 5 fecal leukocytes/high-power field (adjusted OR = 2.8, 95% CI = 1.2-7.0) were significant predictors of detecting an acute inflammatory pathogen by PCR. Use of multiplex PCR enabled the detection of gastrointestinal pathogens in a high proportion of cases, illustrating the utility of this diagnostic tool in epidemiologic studies of infectious diarrhea.

AB - The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015-2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray Gastrointestinal PCR). A target pathogen was identified in 62.7% of subjects. Enteropathogenic Escherichia coli was the most prevalent pathogen and was detected in 24.6% of subjects, followed by Salmonella (22.2%), enteroaggregative E. coli (15.1%), Giardia lamblia (9.5%), and enterotoxigenic E. coli (8.7%). Multiple pathogens were identified in 49.3% of positive stool samples. In a multivariable analysis, the presence of a heart rate ? 90 (adjusted odds ratio [OR] = 3.7, 95% confidence interval [CI] = 1.4-10.0) and > 5 fecal leukocytes/high-power field (adjusted OR = 2.8, 95% CI = 1.2-7.0) were significant predictors of detecting an acute inflammatory pathogen by PCR. Use of multiplex PCR enabled the detection of gastrointestinal pathogens in a high proportion of cases, illustrating the utility of this diagnostic tool in epidemiologic studies of infectious diarrhea.

UR - http://www.scopus.com/inward/record.url?scp=85005976141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85005976141&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.16-0464

DO - 10.4269/ajtmh.16-0464

M3 - Article

VL - 95

SP - 1330

EP - 1337

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 6

ER -