Multiplex PCR amplification of three microsatellites within the CFTR gene

Núria Morral, Xavier Estivill

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Multiplex PCR amplification has been developed for three highly polymorphic microsatellites (IVS8CA, IVS17BTA, and IVS17BCA) located in intronic regions of the CFTR (cystic fibrosis (CF) transmembrane conductance regulator) gene. The triplex PCR reaction required different concentrations of each pair of primers and labeling of primers in the same reaction. Total informativity is obtained in 90.25% of couples requiring analysis of polymorphisms, and when triplex microsatellite analysis is combined with analysis for the six most common CF mutations in the Spanish population, informativity reaches more than 99%.

Original languageEnglish
Pages (from-to)1362-1364
Number of pages3
Issue number4
Publication statusPublished - Aug 1992


ASJC Scopus subject areas

  • Genetics

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