Multiplex cytokine profiling of initial therapeutic response in patients with chronic hepatitis C virus infection

Harlan Wright, Philip Alex, Thuan Nguyen, Teddy Bader, Ahmet Gurakar, Anthony Sebastian, Liberty Gonzales, Gemma Wallis, Mark Naylor, Igor Dozmorov, Michael Centola, Bakr Nour

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Currently available prognostic tools are inadequate to discern the molecular basis of the heterogenic response in hepatitis C virus (HCV)-infected patients treated with the current standard of therapy. The expression and biological function of immune mediators have been shown to be critical in all phases of the immune response to HCV infection and likely therefore influence host response. Herein, a biometric multiplex serum cytokine assay was utilized to characterize the immunomodulatory effects of host response in 10 HCV patients. Serum levels of 17 cytokines were compared before and after 1 month of treatment and against controls. Overall serum cytokine levels were significantly higher in patients (P < 0.05) than controls. Additionally, viral titers decreased in all patients after 1 month of therapy, as did overall serum cytokine levels in the cohort (P < 0.05). To assess relationships between changes in cytokine levels and changes in viral titer, the cohort was divided into three statistically distinct subgroups based on changes in viral titers. Specific sets of cytokines decreased in each group: decreases in CCL4, interleukin (IL)-2, CXCL8, and IL-1β correlated with the greatest drops in viral titer, decreases in IL-5, granulocyte colony stimulating factor (G-CSF), and CCL4 correlated with moderate drops in viral titer, and only CCL2 correlated with the lowest drops in viral titer. Interestingly, decreases in CCL4 levels correlated with decreases in viral titers in all patients. CCL4 controls leukocyte influx and thus propagates inflammation. In conclusion, these data raise the possibility that characteristic changes in host response modulate the therapeutic response, demonstrating the prognostic power of serum cytokine profiling in chronic HCV.

Original languageEnglish
Pages (from-to)1793-1803
Number of pages11
JournalDigestive Diseases and Sciences
Volume50
Issue number10
DOIs
Publication statusPublished - Oct 2005
Externally publishedYes

Fingerprint

Chronic Hepatitis C
Virus Diseases
Hepacivirus
Cytokines
Serum
Therapeutics
Interleukin-5
Granulocyte Colony-Stimulating Factor
Interleukin-1
Interleukin-2
Leukocytes
Inflammation

Keywords

  • Chemokines
  • Cytotoxic and humoral cytokines
  • Pegylated interferon α ribavirin

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Multiplex cytokine profiling of initial therapeutic response in patients with chronic hepatitis C virus infection. / Wright, Harlan; Alex, Philip; Nguyen, Thuan; Bader, Teddy; Gurakar, Ahmet; Sebastian, Anthony; Gonzales, Liberty; Wallis, Gemma; Naylor, Mark; Dozmorov, Igor; Centola, Michael; Nour, Bakr.

In: Digestive Diseases and Sciences, Vol. 50, No. 10, 10.2005, p. 1793-1803.

Research output: Contribution to journalArticle

Wright, H, Alex, P, Nguyen, T, Bader, T, Gurakar, A, Sebastian, A, Gonzales, L, Wallis, G, Naylor, M, Dozmorov, I, Centola, M & Nour, B 2005, 'Multiplex cytokine profiling of initial therapeutic response in patients with chronic hepatitis C virus infection', Digestive Diseases and Sciences, vol. 50, no. 10, pp. 1793-1803. https://doi.org/10.1007/s10620-005-2940-y
Wright, Harlan ; Alex, Philip ; Nguyen, Thuan ; Bader, Teddy ; Gurakar, Ahmet ; Sebastian, Anthony ; Gonzales, Liberty ; Wallis, Gemma ; Naylor, Mark ; Dozmorov, Igor ; Centola, Michael ; Nour, Bakr. / Multiplex cytokine profiling of initial therapeutic response in patients with chronic hepatitis C virus infection. In: Digestive Diseases and Sciences. 2005 ; Vol. 50, No. 10. pp. 1793-1803.
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