Multiple nuclear factors bind to novel positive and negative regulatory elements upstream of the human MHC class I gene HLA-A11

Odile Blanchet, Claude Gazln, Myriam L'haridon, Zohreh Tatari Calderone, Laurent Degos, Francois Sigaux, Pascale Paul

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Quantitative expression of class I genes varies widely in different tissues, during development and in some neopiastic cells. Regulatory DNA sequences controlling levels of transcription of class I genes have been characterized in the murine and swine promoters. Although some regulatory features appear to be retained in humans, most of them are not evolutionary conserved. In this study, we identify novel DNA sequences and factors which contribute to the regulation of the human HLA-A11 gene. Two activator elements (-155 to -91 and -335 to -206) and one negative element (--172 to -156), distinct from those previously described are mapped within 335 bp upstream of exon 1 of the human HLA-A11 gene. Various nuclear factors bind to these regulatory elements, some of which are cell restricted or interact with evolutionary divergent regulatory sequences of the human class I gene promoter. Two of the five DNA-binding sites characterized in this work bind at least two proteins which compete for the occupancy of their site. The identification of these new regulatory elements provides a more comprehensive basis for the understanding of physiological or pathological modulation of MHC class I transcription in humans.

Original languageEnglish
Pages (from-to)1485-1486
Number of pages2
JournalInternational Immunology
Volume6
Issue number10
DOIs
Publication statusPublished - Oct 1994
Externally publishedYes

Fingerprint

HLA-A11 Antigen
MHC Class I Genes
Genes
Exons
Swine
Binding Sites
DNA

Keywords

  • DNA-binding proteins
  • Gene regulation
  • HLA class I
  • Regulatory elements

ASJC Scopus subject areas

  • Immunology

Cite this

Multiple nuclear factors bind to novel positive and negative regulatory elements upstream of the human MHC class I gene HLA-A11. / Blanchet, Odile; Gazln, Claude; L'haridon, Myriam; Tatari Calderone, Zohreh; Degos, Laurent; Sigaux, Francois; Paul, Pascale.

In: International Immunology, Vol. 6, No. 10, 10.1994, p. 1485-1486.

Research output: Contribution to journalArticle

Blanchet, Odile ; Gazln, Claude ; L'haridon, Myriam ; Tatari Calderone, Zohreh ; Degos, Laurent ; Sigaux, Francois ; Paul, Pascale. / Multiple nuclear factors bind to novel positive and negative regulatory elements upstream of the human MHC class I gene HLA-A11. In: International Immunology. 1994 ; Vol. 6, No. 10. pp. 1485-1486.
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