Multiparametric Analyses of Human PBMCs Loaded Ex Vivo with a Candidate Idiotype Vaccine for HCV-Related Lymphoproliferative Disorders

Annacarmen Petrizzo, Maria Lina Tornesello, Maria Napolitano, Giovanna D'Alessio, Angelo Salomone Megna, Riccardo Dolcetti, Valli de Re, Ena Wang, Franco M. Marincola, Franco M. Buonaguro, Luigi Buonaguro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) has been identified as one of the major risk factors for type II mixed cryoglobulinemia (MC), during the clinical evolution of chronic hepatitis, which may lead to development of B cell non-Hodgkin's lymphoma (NHL). We have previously shown that the candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation and maturation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative healthy control subjects, with production of Th2-type cytokines. Here, the effect of the recombinant IGKV3-20 protein on human peripheral blood mononuclear cells (PBMCs) from HCV-positive subjects, with known blood levels of cryoglobulins, is shown via gene expression profiling analysis combined to multiparameter flow cytometry and multiplex analyses of cytokines.

Original languageEnglish
Article numbere44870
JournalPLoS One
Volume7
Issue number9
DOIs
Publication statusPublished - 18 Sep 2012
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Petrizzo, A., Tornesello, M. L., Napolitano, M., D'Alessio, G., Salomone Megna, A., Dolcetti, R., de Re, V., Wang, E., Marincola, F. M., Buonaguro, F. M., & Buonaguro, L. (2012). Multiparametric Analyses of Human PBMCs Loaded Ex Vivo with a Candidate Idiotype Vaccine for HCV-Related Lymphoproliferative Disorders. PLoS One, 7(9), [e44870]. https://doi.org/10.1371/journal.pone.0044870