Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins

Guillaume Ballihaut, Christophe Pécheyran, Sandra Mounicou, Hugues Preud'homme, Régis Grimaud, Ryszard Lobinski, Guillaume Ballihaut, Régis Grimaud, Ryszard Lobinski

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Speciation of heteroelement-containing proteins essential for human health is one of the most rapidly developing areas in bio-inorganic analytical chemistry (metallomics). We discuss recent advances in mass spectrometry (MS) techniques for detection and identification of selenium (Se)-containing proteins separated by gel electrophoresis (GE). These advances include location of Se-containing bands in 1D GE or spots in 2D GE by laser-ablation inductively coupled plasma MS (LA-ICP-MS) followed by protein identification by matrix-assisted laser-desorption/ionization MS (MALDI-MS) and nano-high-performance liquid chromatography-electrospray-tandem MS (nanoHPLC-ESI-MS2). We highlight the differences with the classical proteomics approach resulting from the presence of the Se atom.

Original languageEnglish
Pages (from-to)183-190
Number of pages8
JournalTrAC - Trends in Analytical Chemistry
Volume26
Issue number3
DOIs
Publication statusPublished - 1 Mar 2007
Externally publishedYes

Fingerprint

Inductively coupled plasma
Laser ablation
Selenium
Electrophoresis
selenium
ablation
Ionization
Mass spectrometry
Desorption
desorption
electrokinesis
ionization
gel
mass spectrometry
laser
Gels
plasma
matrix
protein
Lasers

Keywords

  • Gel electrophoresis
  • Inductively-coupled plasma
  • Laser ablation
  • Mass spectrometry
  • Multimode detection
  • Nano high-performance liquid chromatography
  • Protein
  • Selenium
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins. / Ballihaut, Guillaume; Pécheyran, Christophe; Mounicou, Sandra; Preud'homme, Hugues; Grimaud, Régis; Lobinski, Ryszard; Ballihaut, Guillaume; Grimaud, Régis; Lobinski, Ryszard.

In: TrAC - Trends in Analytical Chemistry, Vol. 26, No. 3, 01.03.2007, p. 183-190.

Research output: Contribution to journalArticle

Ballihaut, Guillaume ; Pécheyran, Christophe ; Mounicou, Sandra ; Preud'homme, Hugues ; Grimaud, Régis ; Lobinski, Ryszard ; Ballihaut, Guillaume ; Grimaud, Régis ; Lobinski, Ryszard. / Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins. In: TrAC - Trends in Analytical Chemistry. 2007 ; Vol. 26, No. 3. pp. 183-190.
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AU - Grimaud, Régis

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