Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS 2 ) in 1D and 2D gel electrophoresis for selenium-containing proteins

Guillaume Ballihaut, Christophe Pécheyran, Sandra Mounicou, Hugues Preud'homme, Régis Grimaud, Ryszard Lobinski, Guillaume Ballihaut, Régis Grimaud

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31 Citations (Scopus)


Speciation of heteroelement-containing proteins essential for human health is one of the most rapidly developing areas in bio-inorganic analytical chemistry (metallomics). We discuss recent advances in mass spectrometry (MS) techniques for detection and identification of selenium (Se)-containing proteins separated by gel electrophoresis (GE). These advances include location of Se-containing bands in 1D GE or spots in 2D GE by laser-ablation inductively coupled plasma MS (LA-ICP-MS) followed by protein identification by matrix-assisted laser-desorption/ionization MS (MALDI-MS) and nano-high-performance liquid chromatography-electrospray-tandem MS (nanoHPLC-ESI-MS 2 ). We highlight the differences with the classical proteomics approach resulting from the presence of the Se atom.

Original languageEnglish
Pages (from-to)183-190
Number of pages8
JournalTrAC - Trends in Analytical Chemistry
Issue number3
Publication statusPublished - 1 Jan 2007



  • Gel electrophoresis
  • Inductively-coupled plasma
  • Laser ablation
  • Mass spectrometry
  • Multimode detection
  • Nano high-performance liquid chromatography
  • Protein
  • Selenium
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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