Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy

Jana Blazkova, Sarthak Gupta, Yudong Liu, Brice Gaudilliere, Edward A. Ganio, Christopher R. Bolen, Ron Saar-Dover, Gabriela K. Fragiadakis, Martin S. Angst, Sarfaraz Hasni, Nima Aghaeepour, David Stevenson, Nicole Baldwin, Esperanza Anguiano, Damien J. Chaussabel, Matthew C. Altman, Mariana J. Kaplan, Mark M. Davis, David Furman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Despite clear differences in immune system responses and in the prevalence of autoimmune diseases between males and females, there is little understanding of the processes involved. In this study, we identified a gene signature of immature-like neutrophils, characterized by the overexpression of genes encoding for several granule-containing proteins, which was found at higher levels (up to 3-fold) in young (20-30 y old) but not older (60 to >89 y old) males compared with females. Functional and phenotypic characterization of peripheral blood neutrophils revealed more mature and responsive neutrophils in young females, which also exhibited an elevated capacity in neutrophil extracellular trap formation at baseline and upon microbial or sterile autoimmune stimuli. The expression levels of the immature-like neutrophil signature increased linearly with pregnancy, an immune state of increased susceptibility to certain infections. Using mass cytometry, we also find increased frequencies of immature forms of neutrophils in the blood of women during late pregnancy. Thus, our findings show novel sex differences in innate immunity and identify a common neutrophil signature in males and in pregnant women.

Original languageEnglish
Pages (from-to)2479-2488
Number of pages10
JournalJournal of Immunology
Volume198
Issue number6
DOIs
Publication statusPublished - 15 Mar 2017
Externally publishedYes

Fingerprint

Systems Analysis
Neutrophils
Pregnancy
Innate Immunity
Sex Characteristics
Genes
Autoimmune Diseases
Pregnant Women
Immune System
Infection
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Blazkova, J., Gupta, S., Liu, Y., Gaudilliere, B., Ganio, E. A., Bolen, C. R., ... Furman, D. (2017). Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy. Journal of Immunology, 198(6), 2479-2488. https://doi.org/10.4049/jimmunol.1601855

Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy. / Blazkova, Jana; Gupta, Sarthak; Liu, Yudong; Gaudilliere, Brice; Ganio, Edward A.; Bolen, Christopher R.; Saar-Dover, Ron; Fragiadakis, Gabriela K.; Angst, Martin S.; Hasni, Sarfaraz; Aghaeepour, Nima; Stevenson, David; Baldwin, Nicole; Anguiano, Esperanza; Chaussabel, Damien J.; Altman, Matthew C.; Kaplan, Mariana J.; Davis, Mark M.; Furman, David.

In: Journal of Immunology, Vol. 198, No. 6, 15.03.2017, p. 2479-2488.

Research output: Contribution to journalArticle

Blazkova, J, Gupta, S, Liu, Y, Gaudilliere, B, Ganio, EA, Bolen, CR, Saar-Dover, R, Fragiadakis, GK, Angst, MS, Hasni, S, Aghaeepour, N, Stevenson, D, Baldwin, N, Anguiano, E, Chaussabel, DJ, Altman, MC, Kaplan, MJ, Davis, MM & Furman, D 2017, 'Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy', Journal of Immunology, vol. 198, no. 6, pp. 2479-2488. https://doi.org/10.4049/jimmunol.1601855
Blazkova, Jana ; Gupta, Sarthak ; Liu, Yudong ; Gaudilliere, Brice ; Ganio, Edward A. ; Bolen, Christopher R. ; Saar-Dover, Ron ; Fragiadakis, Gabriela K. ; Angst, Martin S. ; Hasni, Sarfaraz ; Aghaeepour, Nima ; Stevenson, David ; Baldwin, Nicole ; Anguiano, Esperanza ; Chaussabel, Damien J. ; Altman, Matthew C. ; Kaplan, Mariana J. ; Davis, Mark M. ; Furman, David. / Multicenter systems analysis of human blood reveals immature neutrophils in males and during pregnancy. In: Journal of Immunology. 2017 ; Vol. 198, No. 6. pp. 2479-2488.
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