Mouse phenotyping

Helmut Fuchs, Valérie Gailus-Durner, Thure Adler, Juan Antonio Aguilar-Pimentel, Lore Becker, Julia Calzada-Wack, Patricia Da Silva-Buttkus, Frauke Neff, Alexander Götz, Wolfgang Hans, Sabine M. Hölter, Marion Horsch, Gabi Kastenmüller, Elisabeth Kemter, Christoph Lengger, Holger Maier, Mikolaj Matloka, Gabriele Möller, Beatrix Naton, Cornelia PrehnOliver Puk, Ildikó Rácz, Birgit Rathkolb, Werner Römisch-Margl, Jan Rozman, Rui Wang-Sattler, Anja Schrewe, Claudia Stöger, Monica Tost, Jerzy Adamski, Bernhard Aigner, Johannes Beckers, Heidrun Behrendt, Dirk H. Busch, Irene Esposito, Jochen Graw, Thomas Illig, Boris Ivandic, Martin Klingenspor, Thomas Klopstock, Elisabeth Kremmer, Martin Mempel, Susanne Neschen, Markus Ollert, Holger Schulz, Karsten Suhre, Eckhard Wolf, Wolfgang Wurst, Andreas Zimmer, Martin Hrabě de Angelis

Research output: Contribution to journalReview article

90 Citations (Scopus)

Abstract

Model organisms like the mouse are important tools to learn more about gene function in man. Within the last 20. years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in techniques, thus creating models which mimic human conditions. Due to pleiotropic effects, one gene may have different functions in different organ systems or time points during development. Therefore mutant mouse lines have to be phenotyped comprehensively in a highly standardized manner to enable the detection of phenotypes which might otherwise remain hidden. The German Mouse Clinic (GMC) has been established at the Helmholtz Zentrum München as a phenotyping platform with open access to the scientific community (www.mousclinic.de; [1]). The GMC is a member of the EUMODIC consortium which created the European standard workflow EMPReSSslim for the systemic phenotyping of mouse models (http://www.eumodic.org/ [2]).

Original languageEnglish
Pages (from-to)120-135
Number of pages16
JournalMethods
Volume53
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fuchs, H., Gailus-Durner, V., Adler, T., Aguilar-Pimentel, J. A., Becker, L., Calzada-Wack, J., Da Silva-Buttkus, P., Neff, F., Götz, A., Hans, W., Hölter, S. M., Horsch, M., Kastenmüller, G., Kemter, E., Lengger, C., Maier, H., Matloka, M., Möller, G., Naton, B., ... Hrabě de Angelis, M. (2011). Mouse phenotyping. Methods, 53(2), 120-135. https://doi.org/10.1016/j.ymeth.2010.08.006