Morphometric stability of the corneal subbasal nerve plexus in healthy individuals

A 3-year longitudinal study using corneal confocal microscopy

Cirous Dehghani, Nicola Pritchard, Katie Edwards, Dimitrios Vagenas, Anthony W. Russell, Rayaz Malik, Nathan Efron

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Purpose. We examined the age-dependent alterations and longitudinal course of subbasal nerve plexus (SNP) morphology in healthy individuals. Methods. Laser-scanning corneal confocal microscopy ocular screening, and health and metabolic assessment were performed on 64 healthy participants at baseline and at 12-month intervals for 3 years. At each annual visit, eight central corneal images of the SNP were selected and analyzed using a fully-automated analysis system to quantify corneal nerve fiber length (CNFL). Two linear mixed model approaches were fitted to examine the relationship between age and CNFL, and the longitudinal changes of CNFL over three years. Results. At baseline, mean age was 51.9 ± 14.7 years. The cohort was sex balanced (x2 - 0.56, P - 0.45). Age (t - 1.6, P - 0.12) and CNFL (t - -0.50, P - 0.62) did not differ between sexes. A total of 52 participants completed the 36-month visit and 49 participants completed all visits. Age had a significant effect on CNFL (F[1,33] - 5.67, P - 0.02) with a linear decrease of 0.05 mm/mm2 in CNFL per one year increase in age. No significant change in CNFL was observed over the 36-month period (F[155] - 0.69, P - 0.41). Conclusions. The CNFL showed a stable course over a 36-month period in healthy individuals, although there was a slight linear reduction in CNFL with age. The findings of this study have implications for understanding the time-course of the effect of pathology and surgical or therapeutic interventions on the morphology of the SNP, and serves to confirm the suitability of CNFL as a screening/monitoring marker for peripheral neuropathies.

Original languageEnglish
Pages (from-to)3195-3199
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume55
Issue number5
DOIs
Publication statusPublished - 24 Apr 2014
Externally publishedYes

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Nerve Fibers
Confocal Microscopy
Longitudinal Studies
Surgical Pathology
Peripheral Nervous System Diseases
Linear Models
Healthy Volunteers
Health

Keywords

  • Age
  • Corneal confocal microscopy
  • Natural history
  • Subbasal nerve plexus

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Morphometric stability of the corneal subbasal nerve plexus in healthy individuals : A 3-year longitudinal study using corneal confocal microscopy. / Dehghani, Cirous; Pritchard, Nicola; Edwards, Katie; Vagenas, Dimitrios; Russell, Anthony W.; Malik, Rayaz; Efron, Nathan.

In: Investigative Ophthalmology and Visual Science, Vol. 55, No. 5, 24.04.2014, p. 3195-3199.

Research output: Contribution to journalArticle

Dehghani, Cirous ; Pritchard, Nicola ; Edwards, Katie ; Vagenas, Dimitrios ; Russell, Anthony W. ; Malik, Rayaz ; Efron, Nathan. / Morphometric stability of the corneal subbasal nerve plexus in healthy individuals : A 3-year longitudinal study using corneal confocal microscopy. In: Investigative Ophthalmology and Visual Science. 2014 ; Vol. 55, No. 5. pp. 3195-3199.
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abstract = "Purpose. We examined the age-dependent alterations and longitudinal course of subbasal nerve plexus (SNP) morphology in healthy individuals. Methods. Laser-scanning corneal confocal microscopy ocular screening, and health and metabolic assessment were performed on 64 healthy participants at baseline and at 12-month intervals for 3 years. At each annual visit, eight central corneal images of the SNP were selected and analyzed using a fully-automated analysis system to quantify corneal nerve fiber length (CNFL). Two linear mixed model approaches were fitted to examine the relationship between age and CNFL, and the longitudinal changes of CNFL over three years. Results. At baseline, mean age was 51.9 ± 14.7 years. The cohort was sex balanced (x2 - 0.56, P - 0.45). Age (t - 1.6, P - 0.12) and CNFL (t - -0.50, P - 0.62) did not differ between sexes. A total of 52 participants completed the 36-month visit and 49 participants completed all visits. Age had a significant effect on CNFL (F[1,33] - 5.67, P - 0.02) with a linear decrease of 0.05 mm/mm2 in CNFL per one year increase in age. No significant change in CNFL was observed over the 36-month period (F[155] - 0.69, P - 0.41). Conclusions. The CNFL showed a stable course over a 36-month period in healthy individuals, although there was a slight linear reduction in CNFL with age. The findings of this study have implications for understanding the time-course of the effect of pathology and surgical or therapeutic interventions on the morphology of the SNP, and serves to confirm the suitability of CNFL as a screening/monitoring marker for peripheral neuropathies.",
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AU - Edwards, Katie

AU - Vagenas, Dimitrios

AU - Russell, Anthony W.

AU - Malik, Rayaz

AU - Efron, Nathan

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N2 - Purpose. We examined the age-dependent alterations and longitudinal course of subbasal nerve plexus (SNP) morphology in healthy individuals. Methods. Laser-scanning corneal confocal microscopy ocular screening, and health and metabolic assessment were performed on 64 healthy participants at baseline and at 12-month intervals for 3 years. At each annual visit, eight central corneal images of the SNP were selected and analyzed using a fully-automated analysis system to quantify corneal nerve fiber length (CNFL). Two linear mixed model approaches were fitted to examine the relationship between age and CNFL, and the longitudinal changes of CNFL over three years. Results. At baseline, mean age was 51.9 ± 14.7 years. The cohort was sex balanced (x2 - 0.56, P - 0.45). Age (t - 1.6, P - 0.12) and CNFL (t - -0.50, P - 0.62) did not differ between sexes. A total of 52 participants completed the 36-month visit and 49 participants completed all visits. Age had a significant effect on CNFL (F[1,33] - 5.67, P - 0.02) with a linear decrease of 0.05 mm/mm2 in CNFL per one year increase in age. No significant change in CNFL was observed over the 36-month period (F[155] - 0.69, P - 0.41). Conclusions. The CNFL showed a stable course over a 36-month period in healthy individuals, although there was a slight linear reduction in CNFL with age. The findings of this study have implications for understanding the time-course of the effect of pathology and surgical or therapeutic interventions on the morphology of the SNP, and serves to confirm the suitability of CNFL as a screening/monitoring marker for peripheral neuropathies.

AB - Purpose. We examined the age-dependent alterations and longitudinal course of subbasal nerve plexus (SNP) morphology in healthy individuals. Methods. Laser-scanning corneal confocal microscopy ocular screening, and health and metabolic assessment were performed on 64 healthy participants at baseline and at 12-month intervals for 3 years. At each annual visit, eight central corneal images of the SNP were selected and analyzed using a fully-automated analysis system to quantify corneal nerve fiber length (CNFL). Two linear mixed model approaches were fitted to examine the relationship between age and CNFL, and the longitudinal changes of CNFL over three years. Results. At baseline, mean age was 51.9 ± 14.7 years. The cohort was sex balanced (x2 - 0.56, P - 0.45). Age (t - 1.6, P - 0.12) and CNFL (t - -0.50, P - 0.62) did not differ between sexes. A total of 52 participants completed the 36-month visit and 49 participants completed all visits. Age had a significant effect on CNFL (F[1,33] - 5.67, P - 0.02) with a linear decrease of 0.05 mm/mm2 in CNFL per one year increase in age. No significant change in CNFL was observed over the 36-month period (F[155] - 0.69, P - 0.41). Conclusions. The CNFL showed a stable course over a 36-month period in healthy individuals, although there was a slight linear reduction in CNFL with age. The findings of this study have implications for understanding the time-course of the effect of pathology and surgical or therapeutic interventions on the morphology of the SNP, and serves to confirm the suitability of CNFL as a screening/monitoring marker for peripheral neuropathies.

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KW - Natural history

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