Morphologic characterization of alveolar macrophages from subjects with occupational exposure to inorganic particles

T. Takemura, W. N. Rom, V. J. Ferrans, Ronald Crystal

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Alveolar macrophages recovered by bronchoalveolar lavage from 43 nonsmoking or > 5-yr ex-smoking subjects with occupational exposure to inorganic particles (asbestos, n 1/2 19; silica, n 1/2 10; coal, n 1/2 14) were evaluated by light microscopy and transmission and scanning electron microscopy to determine the morphologic changes resulting in these cells from chronic inorganic particulate inhalation. Alveolar macrophages from dust-exposed subjects, including those who had been free of exposure to particles for more than 1 yr, contained particles of higher proportion than did those of normal unexposed subjects. Most of these particles were located within phagolysosomes. The frequency of multinucleated alveolar macrophages was significantly higher in the dust-exposed groups. Ultrastructural studies showed alterations of the morphologic aspects of the surfaces of alveolar macrophages from the dust-exposed subjects, including increased numbers of rufflings, filopodia, pinocytotic vesicles, subplasmalemmal linear densities, and increased frequency of macrophage-macrophage and macrophage-lymphocyte interactions. Furthermore, the numbers of lysosomes were significantly increased in alveolar macrophages from the dust-exposed subjects. Together, these morphologic changes are consistent with the sequelae of phagocytosis, and they emphasize both the role of alveolar macrophages in eliminating inorganic particles from the alveolar spaces and the consequences this role has in alveolar macrophage activation.

Original languageEnglish
Pages (from-to)1674-1685
Number of pages12
JournalAmerican Review of Respiratory Disease
Volume140
Issue number6
Publication statusPublished - 1 Dec 1989
Externally publishedYes

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Alveolar Macrophages
Occupational Exposure
Dust
Macrophages
Phagosomes
Scanning Transmission Electron Microscopy
Pseudopodia
Macrophage Activation
Coal
Asbestos
Bronchoalveolar Lavage
Lysosomes
Phagocytosis
Silicon Dioxide
Inhalation
Microscopy
Smoking
Lymphocytes
Light

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Morphologic characterization of alveolar macrophages from subjects with occupational exposure to inorganic particles. / Takemura, T.; Rom, W. N.; Ferrans, V. J.; Crystal, Ronald.

In: American Review of Respiratory Disease, Vol. 140, No. 6, 01.12.1989, p. 1674-1685.

Research output: Contribution to journalArticle

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